Safe PCR-Pure Kit

 3,810,000 6,858,000

Safe PCR-Pure Kit provides a rapid, precise and convenient method for efficient nucleic acid purification. The procedure is based on spin column technology which takes less than 10 minutes. Special buffers provided with kit are enhanced for well-organized DNA recovery and contaminants removal. Due to presence of high concentration of salts, DNA adsorbs to the silica membrane and contaminants pass through the column. Finally, DNA is solved by ROJE rehydration buffer. Typical yield, depends on density of starting material. The isolated nucleic acid is ready to use in downstream applications such as restriction endonuclease digestions.

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Description

Safe PCR-Pure Kit content

Component 50 preps 100 preps
RBB 15 ml 30 ml
GWB 9 ml 18 ml
RSB 5 ml 10 ml
Spin column 50 100
Collection tube 50 100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

Safe PCR-Pure Kit provides the components and procedures necessary for purifying PCR product. Notice, Kits are intended for molecular biology applications not for diagnostic use.  We recommend all users to study DNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

Safe PCR-Pure Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Equipment & Reagents to be Supplied by User

  • Absolute Ethanol
  • Pipets and pipet tips
  • 5 ml Microcentrifuge tube
  • Vortex
  • Microcentrifuge
  • Dry Heat block / Water Bath

Applications

The isolated DNA can be used in many downstream applications:

  • Sequencing
  • Restriction digestion
  • Cloning

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

100 reactions, 50 reactions

Specification

Features Specifications
Elution volume 30-50 µl
Technology Silica technology
Main sample type PCR products
Processing            Manual
Operation time per reaction Less than 10 min
Typical Yield Up to 10 µg
Average purity A260/A280= 1.7-2.0

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Protocol

none!

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Resources

MSDS Safe PCR-Pure Kit

Hand Book Safe PCR-Pure Kit

Quick Protocol EN  FA

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