PlasJia Kit

Description

Kit Content

Component	50 preps
SOB	12.5ml
PLB	12.5ml
NOT	17.5ml
PWB (Concentrated)	12.5ml
PEB	5ml
Prime RNase A	1.5mg
HiPure DR Column Column	50
Collection tube	100

 

Storage

PlasJia Mini Plasmid Extraction Kit is stable for 1 years when stored in a constant temperature 15 ~ 25℃. Prime RNase A is stable when stored at -15°C or -20°C.

 

Intended Use

The PlasJia Mini Plasmid Extraction Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. The PlasJia Mini Plasmid Extraction Kit is designed for rapid and cost-effective small-scale preparation of high-quality plasmid DNA from recombinant bacterial cultures. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. Each spin column can recover up to 20 µg of plasmid DNA. The kit can be successfully used for efficient purification of any size plasmids and cosmids. The actual plasmid yield and optimal culture volume depend on the plasmid copy number and medium used for cultivation.

 

Guarantee and Warranty

ROJETechnologies guarantee the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If products do not meet your satisfaction, please contact the technical support team for reasons other than misuse. If the problem is due to the manufacturing process, the ROJE team will replace the kit for you.

 

Notice to Purchaser 

This product is only for experimental and not for commercial use of any kind. There is no right to resell the kit or any of its components. For information about our licensing or distributors, contact the ROJE business team.

 

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

* Buffers PLB and NOT contain chaotropic salts which are irritants and not compatible with disinfecting agents containing bleach. Take appropriate laboratory safety measures and wear gloves when handling.

If precipitate forms in the buffers during storage, it should be redissolved by incubating the buffers at 37°C before use.

After addition of Prime RNase A, the Resuspension Solution can be used for 6 months when stored at 4°C.

Note: Close the bag with Spin Columns tightly after each use!

 

Quality control

PlasJia Mini Plasmid Extraction Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

 

Description

PlasJia Mini Plasmid Extraction Kit offers a simple, rapid and cost-effective method for purification of high copy and low copy plasmid DNA from bacterial cells. This kit is designed for the preparation of up 20 μg of high-purity plasmid DNA from 1 ~ 5 ml overnight E. coli culture in LB medium. Plasmid DNA purified with mini kit is immediately ready for use.

 

Procedure

In PlasJia Kit, pelleted bacterial cells are resuspended and subjected to SOB and PLB lysis buffers. The resulting lysate is neutralized with NOT Buffer to create appropriate conditions for the binding of plasmid DNA on the silica membrane in the spin column. Cell debris and SDS precipitate are pelleted by centrifugation, and the supernatant containing the plasmid DNA is loaded onto the spin column membrane. The adsorbed DNA is washed with PWB buffer to remove contaminants and is then eluted with a small volume of the PEB buffer. The purified plasmid DNA is ready for immediate use in all molecular biology procedures such as conventional digestion with restriction enzymes, PCR, transformation, and automated sequencing.

Equipment & Reagents to Be Supplied by User

• Ethanol (96-100%)
• Pipets and pipet tips
• 5 ml Microtube
• Vortex
• Centrifuge
• Micro centrifuge

 

Applications

The isolated DNA can be used in many downstream applications:

• Different kinds of PCRs, including Long-range PCR
• Sequencing
• Restriction digestion
• Southern blotting
• Cloning
• Ligation
• Transformation

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