Description
Kit content
| Component | 50 preps | 100 preps |
| TLB | 9 ml | 18 ml |
| GLB | 12.5 ml | 25 ml |
| BWB1 (concentrate) | 17 ml | 35 ml |
| BWB2 (concentrate) | 15 ml | 32 ml |
| RRB | 10 ml | 20 ml |
| RJ-Protease | 1.25 ml | 2 X 1.25 ml |
| HiPure DR Column | 50 | 100 |
| Collection Tube | 100 | 200 |
Storage
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.
Intended Use
DNall Plus Kit provides the components and procedures necessary for purifying genomic DNA from blood, buffy coat, body fluid, buccal cells, animal tissue, serum and plasma, fixed tissue, cultured cells and bacteria. Notice that, DNall Plus Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com
Quality Control
DNall Plus Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com
Procedure
DNall Plus Kit is designed for isolating DNA from various sample types. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and ROJE specific lysis buffer. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of chaotropic salts and Ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure genomic DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.7–2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.
Equipment & Reagents to be supplied by user
- Ethanol (96-100%)
- Pipets and pipet tips
- 5 ml Microtube
- Vortex
- Centrifuge
- Micro centrifuge
- Dry Heat Block/ Water Bath
Applications
The isolated DNA can be used in many downstream applications:
- Different kinds of PCRs, including Long-range PCR
- Sequencing
- Restriction digestion
- Southern blotting
- Cloning
Recommended starting material:
To reach optimized results it is better to follow as listed here. The size of recommended material is written in Table 2.
Table 2: Appropriate size of starting material
| Sample | Size of Starting Material |
| Blood* | Up to 250 µl |
| Buffy coat | Up to 200 µl |
| Animal tissue | All tissue kind expect spleen: up to 25 mg
(spleen up to 10 mg) |
| Bacteria cells | 2.5 x 10 8 to 2 x 109 |
| Cultured cell | ≤5 x 106 cells |
*White Blood Cell counts are extremely variable from a person to person and therefore, for optimum results its recommended to determine cell counts for choosing the best protocol for purification. For healthy individuals, white blood cell counts normally from 4-10 million per 1ml blood; however, depending on disease state, cell counts may be higher or lower than the normal range.
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, [email protected]
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