Gel & PCR Purification Set

 10,400,000 18,500,000

Gel and PCR Purification Set provides a rapid, careful and convenient method for both efficient nucleic acid isolation from agarose gel and nucleic acid purification. The procedure is based on HiPure DR Column technology, which takes less than 30 minutes for gel purification and less than 15 minutes for PCR purification. Special buffers provided with the kit are enhanced for well-organized DNA recovery and contaminants removal. Due to presence of high concentration of salts, DNA adsorbs to the silica membrane and contaminants pass through the column. Finally, DNA is solved in prepared ROJE rehydration buffer. Typical yield depends on density of the bands on gel. The isolated nucleic acid is ready to use in downstream applications such as restriction endonuclease digestions.

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Description

Gel & PCR Purification Set content

Component 50 preps 100 preps
RGB  30 ml 60 ml
RPB 28 ml 2 x 28 ml
GWB 10 ml 2 x 10 ml
RSB 5 ml 10 ml
HiPure DR column  50 100
Collection tube 50 100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

Gel and PCR Purification Set provides the components and procedures necessary for purifying genomic DNA, PCR product from gel and PCR product purification. Notice, Gel and PCR Purification Set is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.

 Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

Gel and PCR Purification Set is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

Gel and PCR Purification Set provides a rapid, careful and convenient method for both efficient nucleic acid isolation from agarose gel and nucleic acid purification. The procedure is based on HiPure DR Column technology, which takes less than 30 minutes for gel purification and less than 15 minutes for PCR purification. Special buffers provided with the kit are enhanced for well-organized DNA recovery and contaminants removal. Due to presence of high concentration of salts, DNA adsorbs to the silica membrane and contaminants pass through the column. Finally, DNA is solved in prepared ROJE rehydration buffer. Typical yield depends on density of the bands on gel. The isolated nucleic acid is ready to use in downstream applications such as restriction endonuclease digestions.

 Equipment & Reagents to Be Supplied by User

  • Absolute ethanol
  • Ethanol (96-100%)
  • Pipets and pipet tips
  • 1.5 ml Microcentrifuge tube
  • Vortex
  • Centrifuge
  • Microcentrifuge
  • Dry Heat Block / Water Bath

 

Applications The isolated nucleic acid can be used in many downstream applications:

  • Sequencing
  • Restriction digestion
  • Cloning

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

100 reactions, 50 reactions

Specification

Features Specifications
Elution volume 30-50 µl
Technology Silica technology
Main sample type DNA bands on agarose gel, PCR Product
Processing              Manual
Sample amount ·         Up to 200 mg agarose gel

·         For PCR product varied

Operation time per reaction ·         Less than 30 min (for Gel Purification)

·         Less than 15 min (for PCR Product)

Typical yield ·         Up to 10 µg (for Gel Purification)

·         15 µg for PCR Product

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Protocol

none!

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Resources

MSDS Gel & PCR Purification Set Kit

Hand Book Gel & PCR Purification Set Kit

Quick Protocol EN  FA

Video Protocol Download

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