DNJia Micro Kit

DNJia Micro Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic and Cell Free DNA isolation from body fluid and amniotic fluid. The procedure is based on spin column technology, which takes less than 30 minutes. DNJia Micro Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. Typical yields of genomic DNA vary, depending on cell density of samples. The isolated DNA is ready to use in downstream applications such as PCR and rt-PCR analysis.

Category: SKU: DN013070 Tag:

Description

Kit Content

Component 100 preps
GLB 60 ml
BWB1 (concentrate) 2 X 16 ml
BWB2 (concentrate) 2 X 16.5 ml
RRB 20 ml
RJ-Protease 4 x 1.5 ml
Micro Column 100
Collection Tube 300
Carrier RNA 620 μg

 

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested storing RJ-Protease at -20°C for longer stability; However, for routine use, it is recommended that RJ-Protease aliquoted to 100µl volumes before storage at 2-8°C and avoid frequent freeze-thaw. Also, Carrier RNA is storable at room temperature before preparation. However, after adding RRB buffer, it is recommended that it must store at -20°C and be aliquoted to 100µl volumes and avoid frequent freeze-thaw. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

 

Intended Use

DNJia Micro Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic and Cell Free DNA isolation from body fluid and amniotic fluid. The procedure is based on spin column technology, which takes less than 30 minutes. DNJia Micro Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. Typical yields of genomic DNA vary, depending on cell density of samples. The isolated DNA is ready to use in downstream applications such as PCR and rt-PCR analysis.

 

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

 

Notice to Purchaser

This product is only for experiments and not for commercial use in any kind. No right to resell this kit or any components. For information about our licensing or distributors contact ROJE business team.

 

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

 

Quality Control

DNJia Micro Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

 

Description

DNJia Micro Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic and Cell Free DNA isolation from body fluid and amniotic fluid. The procedure is based on spin column technology, which takes less than 30 minutes. DNJia Micro Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. Typical yields of genomic DNA vary, depending on cell density of samples. The isolated DNA is ready to use in downstream applications such as PCR analysis and restriction endonuclease digestions.

 

Procedure

DNJia Micro Kit is designed for isolating DNA from 600μl-6ml body fluid and amniotic fluid. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and GLB. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specifics washing buffers. Pure DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/A 280 ratios of 1.7-2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

 

Equipment & Reagents to Be Supplied by User

  • Ethanol (96-100%)
  • Pipets and pipet tips
  • 1.5ml Microtube
  • Vortex
  • Centrifuge
  • Micro centrifuge
  • Dry Heat Block/ Water Bath

 

Applications

The isolated DNA can be used in many downstream applications:

  • Different kinds of PCRs, including Long-range PCR
  • Sequencing
  • Restriction digestion
  • Southern blotting

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Specification

Features Specifications
Elution volume 25-50 µl
Technology Silica technology
Main sample type Amniotic fluid-body fluid
Processing Manual
Sample amount Up to 600 μl- 6ml
Operation time per reaction Less than 30 Min
Typical yield Varies
Average purity A260/A280= 1.7-2.0
Size of DNA purified Up to 50 Kb
Enzyme RJ-Protease

 

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Resources

Hand Book Download

Quick Protocol Download

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