Description
Kit Content
Component | 100 preps |
GLB | 60 ml |
BWB1 (concentrate) | 2 X 16 ml |
BWB2 (concentrate) | 2 X 16.5 ml |
RRB | 20 ml |
RJ-Protease | 4 x 1.5 ml |
Micro Column | 100 |
Collection Tube | 300 |
Carrier RNA | 620 μg |
Storage
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested storing RJ-Protease at -20°C for longer stability; However, for routine use, it is recommended that RJ-Protease aliquoted to 100µl volumes before storage at 2-8°C and avoid frequent freeze-thaw. Also, Carrier RNA is storable at room temperature before preparation. However, after adding RRB buffer, it is recommended that it must store at -20°C and be aliquoted to 100µl volumes and avoid frequent freeze-thaw. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.
Intended Use
DNJia Micro Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic and Cell Free DNA isolation from body fluid and amniotic fluid. The procedure is based on spin column technology, which takes less than 30 minutes. DNJia Micro Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. Typical yields of genomic DNA vary, depending on cell density of samples. The isolated DNA is ready to use in downstream applications such as PCR and rt-PCR analysis.
Guarantee & Warranty
ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.
Notice to Purchaser
This product is only for experiments and not for commercial use in any kind. No right to resell this kit or any components. For information about our licensing or distributors contact ROJE business team.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.
Quality Control
DNJia Micro Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.
Description
DNJia Micro Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic and Cell Free DNA isolation from body fluid and amniotic fluid. The procedure is based on spin column technology, which takes less than 30 minutes. DNJia Micro Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. Typical yields of genomic DNA vary, depending on cell density of samples. The isolated DNA is ready to use in downstream applications such as PCR analysis and restriction endonuclease digestions.
Procedure
DNJia Micro Kit is designed for isolating DNA from 600μl-6ml body fluid and amniotic fluid. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and GLB. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specifics washing buffers. Pure DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/A 280 ratios of 1.7-2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.
Equipment & Reagents to Be Supplied by User
- Ethanol (96-100%)
- Pipets and pipet tips
- 1.5ml Microtube
- Vortex
- Centrifuge
- Micro centrifuge
- Dry Heat Block/ Water Bath
Applications
The isolated DNA can be used in many downstream applications:
- Different kinds of PCRs, including Long-range PCR
- Sequencing
- Restriction digestion
- Southern blotting
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