|BWB1 (concentrate)||35 ml|
|BWB2 (concentrate)||16.5 ml|
|RJ-Protease||2 x 1.25 ml|
|Carrier RNA||610 μg|
|HiPure VI Column||100|
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease and Carrier RNA at -20 °C for longer stability; however, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.
DNall VirAll Kit provides the components and procedures necessary for purifying viral DNA/RNA from cell-free samples such as body fluid, serum, plasma and animal tissue. Notice that, DNall VirAll Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies
The Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies
This kit is designed for isolating both DNA and RNA from body fluid, serum, plasma and animal tissue. Lysis is achieved by incubation of the sample in GLB, for both DNA and RNA isolation; and in a RJ-Protease enzyme solution, just for viral DNA isolation. Appropriate conditions for DNA and RNA binding to the silica membrane is achieved by the addition of Ethanol to the lysate. Then, DNA or RNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure viral DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has a symmetric peak at 260 nm by spectrophotometer, confirms high purity.
Equipment & Reagents to be supplied by user
- Ethanol (96-100%)
- Sterile, RNase-free pipets and pipet tips
- 5 ml Microtube
- Centrifuge and Micro centrifuge
- Dry Heat Block/ Water Bath
The isolated DNA/RNA can be used in many downstream applications:
- Different kinds of PCRs
- Restriction enzyme digestion
- Viral genotyping
- Viral detection
- Viral load monitoring
Recommended starting material:
The size of recommended starting material to use with determined lysis volume are listed here.
Table 2: Recommended starting material and Lysis Buffer amount
|Sample||Size of Starting material||Lysis Buffer Amount|
|Plasma/serum||Up to 200 µl||200 µl|
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, Technicalsupport@Rojetechnologies.com