DNall VirAll Kit

 35,000,000

The DNall VirAll Kit is designed for rapid and efficient purification of high-quality viral DNA/RNA from various human and animal liquid samples such as body fluid, plasma, serum and animal tissue. The kit utilizes a silica-based membrane technology in the form of a convenient spin column for nucleic acid isolation. DNall VirAll Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA/RNA can be directly used in PCR, qPCR or other nucleic acid-based assays.

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Description

Kit content

Component 100 preps
GLB 25 ml
TLB 18 ml
BWB1 (concentrate) 2 x 16 ml
BWB2 (concentrate) 2 x 12 ml
ERR 20 ml
RJ-Protease 2 x 1.25 ml
Carrier RNA 620  μg
HiPure DR Column 100
Collection Tube 100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature except RJ-Protease. Carrier RNA also can be store at room temperature, however after preparation; it should be divided into conveniently sized aliquots and stored at -20 °C. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

DNall VirAll Kit provides the components and procedures necessary for purifying viral DNA/RNA from cell-free samples such as body fluid, serum, plasma and animal tissue. Notice that, DNall VirAll Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

DNall VirAll Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at www.rojetechnoloes.com.

Description

The DNall VirAll Kit is designed for rapid and efficient purification of high-quality viral DNA/RNA from various human and animal liquid samples such as body fluid, plasma, serum and animal tissue. The kit utilizes a silica-based membrane technology in the form of a convenient spin column for nucleic acid isolation. DNall VirAll Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA/RNA can be directly used in PCR, qPCR or other nucleic acid-based assays.

Procedure

DNall VirAll Kit is designed for isolating both viral DNA and RNA from body fluid, serum, plasma and genomic DNA from animal tissue. Lysis is achieved by incubation of the sample in GLB, for both DNA and RNA isolation; and in a RJProtease enzyme solution, just for viral DNA isolation. Appropriate conditions for DNA and RNA binding to the silica membrane is achieved by the addition of ethanol to the lysate. Then, DNA or RNA is selectively bound to the membrane. 7 Contaminants are removed by two specific washing buffers. Pure viral DNA/RNA is finally eluted in rehydration buffer. Isolated DNA/RNA is ready to use in downstream applications. A symmetric peak at 260 nm by spectrophotometer, confirms high purity of isolated nucleic acid.

Equipment & Reagents to Be Supplied by User

  • Molecular biology grade ethanol (96-100%)
  • Sterile, RNase-free pipets and pipet tips
  • 1.5 ml Microtube
  • Vortex
  • Centrifuge and Micro centrifuge
  • Dry Heat Block/ Water Bath

Applications

The isolated DNA/RNA can be used in many downstream applications:

  • Different kinds of PCRs
  • Viral genotyping
  • Viral detection
  • Viral load monitoring

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

Additional information

Size

100 reactions

Specification

Features Specifications
Elution volume 20-200 µl
Technology Silica technology
Main sample type Body Fluid, serum, Plasma, Animal tissue
Processing  Manual
Sample amount • Plasma and serum: Up to 200 µl
• All tissue kind expect spleen: up to 25 mg (spleen up to 10 mg)
Biomolecule isolation DNA and RNA
Operation time per reaction Less than 30 min (for viral DNA/RNA isolation)
• Less than 20 Min (for whole blood, buffy coat, serum and plasma)
• Less than 2 h (for animal tissue and bacteria)
Typical yield Varies
Carrier RNA Provided in the kit
Enzyme RJ-Protease

 

Protocol

none!

Resources

MSDS Download

Quick Protocol EN  FA

Video Protocol Download

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