DNall VirAll Kit


The DNall VirAll Kit is designed for rapid and efficient purification of high-quality viral DNA/RNA from various human and animal liquid samples such as body fluid, plasma, serum and animal tissue. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, which takes less than 30 minutes. DNall VirAll Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA/RNA can be directly used in PCR, qPCR or other nucleic acid-based assays.

Category: SKU: N/A


Kit content

Component 100 preps
GLB 25 ml
TLB 18 ml
BWB1 (concentrate) 35 ml
BWB2 (concentrate) 16.5 ml
ERR 20 ml
RJ-Protease 2 x 1.25 ml
Carrier RNA 610  μg
HiPure VI Column 100
Collection Tube 100


Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease and Carrier RNA at -20 °C for longer stability; however, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use 

DNall VirAll Kit provides the components and procedures necessary for purifying viral DNA/RNA from cell-free samples such as body fluid, serum, plasma and animal tissue. Notice that, DNall VirAll Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies

Quality Control

The Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies


This kit is designed for isolating both DNA and RNA from body fluid, serum, plasma and animal tissue. Lysis is achieved by incubation of the sample in GLB, for both DNA and RNA isolation; and in a RJ-Protease enzyme solution, just for viral DNA isolation. Appropriate conditions for DNA and RNA binding to the silica membrane is achieved by the addition of Ethanol to the lysate. Then, DNA or RNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure viral DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

Equipment & Reagents to be supplied by user

  • Ethanol (96-100%)
  • Sterile, RNase-free pipets and pipet tips
  • 5 ml Microtube
  • Vortex
  • Centrifuge and Micro centrifuge
  • Dry Heat Block/ Water Bath


The isolated DNA/RNA can be used in many downstream applications:

  • Different kinds of PCRs
  • Restriction enzyme digestion
  • Viral genotyping
  • Viral detection
  • Viral load monitoring

Recommended starting material:

The size of recommended starting material to use with determined lysis volume are listed here.

Table 2: Recommended starting material and Lysis Buffer amount

Sample Size of Starting material Lysis Buffer Amount
Plasma/serum Up to 200 µl 200 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]


Additional information


100 reactions


Features Specifications
Elution volume 20-150 µl
Technology Silica technology
Main sample type Body Fluid, serum, Plasma, Animal tissue
Processing  Manual
Sample amount Up to 250 µl
Biomolecule isolation DNA and RNA
Operation time per reaction Less than 30 Min
Typical yield Varies
Carrier RNA Not provided in the kit
Enzyme RJ-Protease




MSDS Download

Quick Protocol EN  FA

Video Protocol Download


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