Viga SARS-CoV-2 and Influenza A/B

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The Viga SARS COV-2 and Influenza A/B Molecular Diagnostic Kit is based on one step RT-PCR reverse transcriptase reaction. A part of the RNA sequence of Pathogen is converted to cDNA and then used as a PCR reaction template.

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Description

Kit content

Components 100 Preps
Q-ROMAX, 4X 500 µl
Pro II Mix 400 µl
RTase,      Recombinant

Reverse Transcriptase, RNase H-

100 µl
Positive Control 150 µl
Negative control 150 µl

Description

The Viga SARS COV-2 and Influenza A/B Molecular Diagnostic Kit is based on one step RT-PCR reverse transcriptase reaction. A part of the RNA sequence of Pathogen is converted to cDNA and then used as a PCR reaction template. The resulting PCR product is identified by an oligonucleotide probe labeled with fluorescent color. This Kit detects N gene from SARS COV-2 as well as M2 gene of the Influenza A virus, and NS1 gene of Influenza B virus. Other Coronaviruses and other strains of Influenza virus are not identified with this Kit.

Specimen collection

 Consider all samples potentially infectious and transfer them by precisely following the biosafety guidelines. The collection swab should have a synthetic tip, such as nylon or dacron, and an aluminum or plastic shaft. cotton swab with wooden shafts is not recommended. After sample collection, swabs should be stored at VTM (virus transfer medium) immediately.

Specimen isolation

For viral nucleic acid isolation use (REF: RN983072), RNJia Virus Kit or other kits approved by the ministry of health.

 Process

Take out each component from the kit and place them on bench top. Allow the reagents to equilibrate to room temperature, then briefly vortex each tube for later use. The volume of isolated sample in this test should be 10µl. Prepare PCR reaction refer to Table 1 and then perform Realtime PCR refer to Table 2.

Table1:PCR reaction preparation

Components volume
Q-ROMAX, 4X µl 5
RTase, Recombinant Reverse

Transcriptase

µl 1
Pro II Mix µl 4
Isolated RNA µl 10

Table 2: One-step multiple Real time RT-PCR

Cycle  Temp time Stage
1 50ºC 20min cDNA

synthesis

1 95ºC 3min Polymerase activation
 

 

 

45

95ºC 10min Denaturation
55ºC 45min Binding and amplification of nucleic

acid

72ºC 15min Final

amplification

Interpretation of results

  • To analyze the PCR results, select FAM channel for Influenza A, Yakima Yellow channel for Influenza B virus, Texas Red channel for SARS CoV-2 and cy5 channel for RNase P gene.
  • Please check both amplification curves and Cqfor each sample. The linear and logarithmic diagrams of the sample should both be checked and compared to the negative control.
  • Evaluation of results should be done after reviewing positive and negative controls and confirming their acceptance. If control result is not acceptable, the patient’s result cannot be interpreted.
  • Changes in Cq values in positive control may indicate partial inhibition of PCR.

Limitation

  • The optimal performance of this test also depends on how samples are collected, transferred and stored.
  • This kit is suitable for diagnosing target viruses in swab samples and respiratory sputum. A negative test does not reject the possibility of SARS CoV-2, Influenza A or B virus, because test result may be affected by sample collection, user error, how the sample is mixed or low virus titration that can be less than the sensitivity of the Kit.
  • The presence of PCR inhibitors can cause false negative results.
  • Sequence diversity in the target area of unknown types of viruses may lead to false negative results or less Kit sensitivity. In these cases, the results should be interpreted based on clinical findings and other tests.
  • The limit of detection in Viga SARS CoV-2 and Influenza A/B Molecular Diagnostic Kit is 200 copies per ml for COVID-19 and 150 copies per ml for Influenza A/B, respectively.

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

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Additional information

Size

100 prep

Resources

Hand Book Viga SARS-CoV-2 and Influenza A/B

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