DNall Pro Kit

 17,500,000 33,000,000

DNall Pro Kit provides a rapid, careful, convenient method for high quality genomic DNA isolation from various sample types including blood, buffy coat, body fluid, buccal cells, animal tissue, serum, plasma, fixed tissue, cultured cells and bacteria. This kit is based on spin column technology for isolation of concentrated, highly purified, intact genomic DNA which is suitable to use for variety of downstream processes such as PCR analysis, restriction endonuclease digestions, Southern blot, genotyping and etc.

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Description

Kit Content

Component 50 preps 100 preps
TLB 9 ml 18 ml
GLB 10 ml 20 ml
BWB1 (concentrate) 16 ml 2 x 16 ml
BWB2 (concentrate) 2 x 16 ml 4 x 16 ml
RRB 10 ml 20 ml
RJ-Protease 2 x 1 ml 4 x 1 ml
HiPure DR Column 50 100
Collection Tube 100 200

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

 Intended Use

DNall Pro Kit provides the components and procedures necessary for purifying genomic DNA from blood, buffy coat, body fluid, buccal cells, animal tissue, serum and plasma, fixed tissue, cultured cells and bacteria. Notice that, DNall Pro Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

DNall Pro Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

DNall Pro Kit provides a rapid, careful, convenient method for high quality genomic DNA isolation from various sample types including blood, buffy coat, body fluid, buccal cells, animal tissue, serum, plasma, fixed tissue, cultured cells and bacteria. This kit is based on spin column technology for isolation of concentrated, highly purified, intact genomic DNA which is suitable to use for variety of downstream processes such as PCR analysis, restriction endonuclease digestions, Southern blot, genotyping and etc.

Procedure

DNall Pro Kit is designed for isolating DNA from various sample types. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and ROJE specific lysis buffer. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of chaotropic salts and ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure genomic DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.7–2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

Equipment & Reagents to Be Supplied by User

  • Ethanol (96-100%)
  • Pipets and pipet tips
  • 5 ml Microtube
  • Vortex
  • Centrifuge
  • Micro centrifuge
  • Dry Heat Block/ Water Bath

Applications

The isolated DNA can be used in many downstream applications:

  • Different kinds of PCRs, including Long-range PCR
  • Sequencing
  • Restriction digestion
  • Southern blotting
  • Cloning

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

Additional information

Size

100 prep, 50 prep

Resources

Hand Book DNall Pro Kit

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