Description

Kit Content

Component	100 preps
GLB	20 ml
BWB1 (concentrate)	2 x 16 ml
BWB2 (concentrate)	4 x 16 ml
RRB	20 ml
RJ-Protease	4 x 1 ml
HiPure DR Column	100
Collection Tube	200

 

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

DNJia Blood and Cell Pro Kit provides the components and procedures necessary for purifying genomic DNA from blood, buffy coat and animal cultured cell. Notice that, DNJia Blood and Cell Pro Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

DNJia Blood and Cell Pro Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

DNJia Blood and Cell Pro Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic DNA isolation from whole blood, buffy coat and animal cultured cell. The procedure is based on spin column   technology,   which takes less than 20 minutes. DNJia Blood and Cell Pro Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. Typical yields of genomic DNA vary, depending on cell density of blood samples. The isolated DNA is ready to use in downstream applications such as PCR analysis and restriction endonuclease digestions.

Procedure

DNJia Blood and Cell Pro Kit is designed for isolating DNA from whole blood, buffy coat, and animal cultured cell. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and GLB. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure genomic DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/A 280 ratios of 1.7-2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

Equipment & Reagents to Be Supplied by User

• Ethanol (96-100%)
• Pipets and pipet tips
• 5 ml Microtube
• Vortex
• Centrifuge
• Micro centrifuge
• Dry Heat Block/ Water Bath

Applications

The isolated DNA can be used in many downstream applications:

 

• Different kinds of PCRs, including Long-range PCR
• Sequencing
• Restriction digestion
• Southern blotting
• Cloning