DNJia CF Kit

 44,400,000

DNJia CF Kit provides a rapid, careful and convenient method for high quality cell free DNA or RNA isolation from Serum and plasma. This kit is based on silica column technology for isolation of concentrated, highly purified, intact genomic DNA which is suitable to use for variety of downstream processes such as PCR analysis, NIPT and Sex determination test.

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Description

Kit Content

Component 50 preps
BFC 2 x 60 ml
RFC (concentrate)  5 x 36 ml
ERW1 (concentrate)  19 ml
ERW2 (concentrate) 14 ml
ERR  10 ml
Carrier RNA  310 μg
RJ-Protease  17×1 ml
HiPure CF Mini Column Set  50

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

DNJia CF Kit provides the components and procedures necessary for purifying cell free DNA or RNA from serum or plasma. This kit has IVD certificate to be used for molecular biology applications. We recommend all users to study DNA experiments guideline, before starting their work.

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

DNJia CF Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

DNJia CF Kit provides a rapid, careful and convenient method for high quality cell free DNA or RNA isolation from Serum and plasma. This kit is based on silica column technology for isolation of concentrated, highly purified, intact genomic DNA which is suitable to use for variety of downstream processes such as PCR analysis, NIPT and Sex determination test.

Procedure

DNJia CF Kit is designed for isolating cell free nucleic acid from serum and plasma. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and ROJE specific lysis buffer. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of chaotropic salts and ethanol to the lysate, then DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure nucleic acid is finally eluted in rehydration buffer. Isolated nucleic acid is ready to use in downstream applications. Purified nucleic acids are free of impurities such as proteins and nucleases.

Equipment & Reagents to Be Supplied by User

  • Ethanol (96-100%)
  • Isopropanol
  • Pipets and pipet tips
  • 1.5 ml Microtube
  • Vortex 6
  • Dry Heat Block/ Water Bath
  • Centrifuge
  • Vacuum device

Applications

The isolated nucleic acid can be used in many downstream applications:

  • PCR analysis
  • NIPT
  • Sex determination test

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

Additional information

Size

50 reactions

Specification

Applications Specifications
Elution volume(µl) 20-150 µl
Technology Silica technology
Main sample type Serum/ plasma/ urine / cell-free body fluids
Processing Manual
Sample amount Up to 3 ml
Operation time per reaction Less than 1 h
Typical yield Vries
Average purity A260/A280= 1.7-2.0
Size of nucleic acid purified <1000 bp (DNA) or <1000 nt (RNA)
Enzyme RJ-Protease
Carrier RNA Provided in the kit

Protocol

none!

Resources

MSDS Download

Hand Book Download

Quick Protocol EN  FA

Video Protocol Download

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