DNSol Midi Kit

Description

DNSol Midi Kit content

Component	50 preps
RBC Lysis Buffer	8 x 50 ml
ROS	2 x 32 ml
PPS	28 ml
RRB	10 ml

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Procedure

In DNSol kits, for DNA isolation from whole blood, WBCs are separated after lysing RBCs, and then they are lysed using special ionic detergents. Contaminants such as protein are precipitated using an efficient salting out procedure; DNA will be precipitated using Absolute ethanol or isopropanol. DNA is washed using 70% ethanol to wash away the salts and then it is rehydrated using freshly made Rehydration Buffer. Purified DNA has A260/A280 ratios approximately 1.8 and A260/A230 ratios of 1.8-2.2. The rehydrated DNA is stable for several months at 4 °C and greater than 1 year at -20 °C.

Intended Use

The DNSol Kit provides the components and procedures necessary for purifying genomic DNA from blood and buffy coat. Notice that, DNSol Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study DNA experiments guideline, before starting their work.

Description

Obtaining high-quality, intact DNA is often the first and most critical step in many fundamental molecular biology applications. Purification is based on thoroughly modified salting out method, designed to reach highly efficient and pure genomic DNA. DNA can be purified in less than 25 minutes. ROJETechnologies kits maximize process efficiency and downstream performance.

Procedure

 In DNSol Kit, for DNA isolation from whole blood, WBCs are separated after lysing RBCs, and then they are lysed using special ionic detergents. Contaminants such as protein are precipitated using an efficient salting out procedure; DNA will be precipitated using absolute ethanol or isopropanol. DNA is washed using 70% ethanol to wash away the salts and then it is rehydrated using freshly made rehydration buffer. Purified DNA has A260/A280 ratios approximately 1.8 and A260/A230 ratios of 1.8-2.2. The rehydrated DNA is stable for several months at 4 °C and greater than 1 year at -20 °C.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies

Quality Control

DNSol Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies

Equipment & Reagents to be supplied by user

• Ethanol (96-100%)
• Pipets and pipet tips
• 5 ml Microtube
• Vortex
• Centrifuge
• Micro centrifuge
• Dry Heat Block/ Water Bath
• Proteinase K / RJ-Protease for DNA isolation from buffy coat

Applications

The isolated DNA can be used in many downstream applications:

• Different kinds of PCRs, including Long-range PCR
• Sequencing
• Restriction digestion
• Southern blotting
• Cloning

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

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