|Component||50 preps||100 preps|
|RLB||50 ml||2 x 50 ml|
|TWB1 (concentrate)||16 ml||2 x 16 ml|
|TWB2 (concentrate)||15 ml||2 x 15 ml|
|Nuclease-free Water||5 ml||10 ml|
|RJ-Protease||1 ml||2 X 1 ml|
|Lysozyme||200 mg||400 mg|
|HiPure DR Column||50||100|
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature (15-25°C). When storage condition is as directed, all reagents are stable until expiration date, indicated on the kit box. All enzymes should be kept at -20 °C for long term storage and for routine use 2-8 °C is suitable.
The RNJia Bacteria Kit provides components and procedures necessary for purifying RNA from Bacteria cells, animal cells, animal tissues and etc. However, it is specialized for RNA isolation from bacteria cells. Notice that, RNJia Bacteria Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies
RNJia Bacteria Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies
RNJia Bacteria Kit Provides a time-saving, reliable and meticulous method for RNA isolation from Bacteria types. RNJia Bacteria Kit is based on spin column technology for isolation of concentrated, highly purified and intact RNA, without use of phenol and chloroform, which is suitable to use for a variety of downstream applications such as Northern blot experiments, Real-time PCR, RNA Sequencing etc.
The RNJia Bacteria Kit is designed for isolating RNA molecules from bacteria. Cells should be lysed and homogenized. Then, RNA binding to the silica membrane is achieved selectively, by the addition of ethanol to the lysate. Contaminants are removed by two specific kinds of washing buffers. Pure RNA is finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream applications. It has A260/A280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.
Equipment & Reagents to Be Supplied by User
- Absolute ethanol
- 1.5 ml RNase free microcentrifuge tubes
- RNase free pipets and pipet tips
- Benchtop microcentrifuge
- Thermoblock or water bath
- Syringe and needles Syringe and needles
The isolated RNA and miRNA can be used in many downstream applications:
- RNA Sequencing
- cDNA synthesis
- RT-PCR and real-time RT-PCR
- Northern, dot blot analysis
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, [email protected]
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