RNall Kit

Description

Kit content

Component	50 preps	100 preps
RNSol Reagent	50 ml	100 ml
RLB	50 ml	2 x 50 ml
TWB1 (concentrate)	16 ml	2 x 16 ml
TWB2 (concentrate)	15 ml	2 x 15 ml
Nuclease-free Water	5 ml	10 ml
HiPure DR Column	50	100
Collection Tube	50	100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol Reagent should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

The RNall Kit provides the components and procedures necessary for purifying RNA and miRNA from cultured cells, animal tissue, PBMC and bacteria cells. Notice that, RNall is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies

Quality Control

RNall Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies.com

Description

RNall kit Provides two time-saving, reliable and meticulous methods for RNA and total RNA (including miRNA) isolation from various sample types including animal tissues (lipid and fibrous), animal cells, whole blood, PBMC and bacteria. However, we suggest using RNJia Bacteria Kit, RNJia Lipid Kit and RNJia Fibrous kits, which are specialized for RNA isolation from bacteria, lipid and fibrous tissues to reach optimum results, while RNall Kit is capable of total RNA isolation (including miRNA) from these sample types if suitable enzymes are purchased. RNall kit is based on silica technology for isolation of concentrated, highly purified and intact total RNA (including miRNA), which is suitable to use for variety of downstream processes such as Northern blot experiments, Real-time PCR, RNA Sequencing, Microarray and etc. In the kit process, two methods are possible: phenol- chloroform based (RNSol H Reagent) and phenol-chloroform free based (RLB). However, it is recommended to use RLB for situations in which the purified RNA will be used in sensitive downstream applications.

Procedure

The RNall kit is designed for isolating all forms of RNA molecules from 0.5-100 mg tissue samples, up to 1×107 animal or bacteria cells and 1-10 ml of whole blood. Fresh or frozen samples should be lysed and homogenized. RLB or RNSol H Reagent buffer can be used as lysis buffer in this step. For procedure in which RNSol H Reagent is used, aqueous phase is separated using chloroform. After lysing samples, the rest of procedure is the same. RNA binding to the silica membrane is achieved selectively, by addition of ethanol to the lysate. Contaminants removed by two specific kinds of washing buffers. Pure RNA and 7 miRNA are finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream amplification. It has A260/A280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

• Absolute ethanol
• 1.5 ml RNase free microcentrifuge tubes
• RNase free pipets and pipet tips
• Benchtop refrigerated microcentrifuge (while RNSol H Reagent is used)
• Vortex
• Thermoblock or water bath
• TissueLyser/ Mortar and pestle (for animal tissue sample)
• Syringe and needles
• Homogenizer

Applications

The isolated RNA and miRNA can be used in many downstream applications:

• RNA Sequencing
• cDNA synthesis
• Poly A+ RNA selection
• Microarrays
• RT-PCR and real-time RT-PCR
• Northern, dot blot analysis

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

This post is also available in: Persian