nRT-ROSET, Recombinant M-MLV

 15,550,000 28,000,000

RTase, Recombinant Reverse transcriptase enzyme is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. The enzyme is purified from E. coli expressing a portion of the gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 8 kb. HiPure-Anchored-Oligo (dT)18 primer is provided in the kit for higher yield and full-length cDNA synthesis.

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Description

Kit content of nRT-ROSET Recombinant 

Component  50 Reactions 100 Reactions
RTase, Recombinant Reverse transcriptase, RNase H-(200U/μl) 55 µl 110 µl
5 x RT-Buffer (specialized for RTase) 220 µl 440 µl
HiPure-Anchored-Oligo (dT)18 Primer (0.5 μg/μl) 55 µl 110 µl
HiPure-Random hexamer primer (0.1 μg/μl) 55 µl 110 µl
HiPure-dNTP mix (10 mM, 2.5 mM each) 110 µl 220 µl
HunteRNase, Recombinant (50 U/μl, just provided in Prime RT-ROSET Kit) 55 µl 110 µl
RT-PCR Grade Water 1.5 ml 1.5 ml

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Intended Use

Notice that, Prime nRT-ROSET and Prime RT-ROSET Kits are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any products do not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Storage

nRT-ROSET Recombinant:  Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Quality Control

Prime nRT-ROSET and Prime RT-ROSET Kits are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

RTase, Recombinant Reverse transcriptase enzyme is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. The enzyme is purified from E. coli expressing a portion of the gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 8 kb. HiPure-Anchored-Oligo (dT)18 primer is provided in the kit for higher yield and full-length cDNA synthesis.

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

100 reactions, 50 reactions

Specification

Features Specifications
Enzyme Reverse transcriptase
Master mix No
Reaction type cDNA synthesis
Sample RNA
Single / Multiplex Single

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Protocol

none!

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Resources

MSDS nRT-ROSET Kit

Hand Book nRT-ROSET Kit

Quick Protocol EN  FA

Video Protocol Download

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