Description
Kit content of nRT-ROSET Recombinant
| Component | 50 Reactions | 100 Reactions |
| RTase, Recombinant Reverse transcriptase, RNase H-(200U/μl) | 50 µl | 100 µl |
| 5 x RT-Buffer (specialized for RTase) | 200 µl | 400 µl |
| HiPure-Anchored-Oligo (dT)18
Primer (0.5 μg/μl) |
50 µl | 100 µl |
| HiPure-Random hexamer primer
(0.1 μg/μl) |
50 µl | 100 µl |
| HiPure-dNTP mix (10 mM, 2.5 mM each) | 100 µl | 200 µl |
| HunteRNase-P, Recombinant (50 U/μl)
(just provided in RT-ROSET Kit) |
50 µl | 100 µl |
| RT-PCR Grade Water | 1.5 ml | 1.5 ml |
Storage
nRT-ROSET Recombinant: Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.
Intended Use of nRT-ROSET Recombinant
Notice that, nRT-ROSET and RT-ROSET Kits are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com
Quality Control
nRT-ROSET and RT-ROSET Kits are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com
Equipment & Reagents to Be Supplied by User
- Pipets and pipet tips
- Microcentrifuge tube
- Thermal cycler
- Mineral oil (for thermal cyclers without a heated lid)
- Primers (If want to synthesis a specific kind of cDNA not total cDNA)
Synthesize First-Strand cDNA
A 20-μL reaction volume can be used for 50 ng–5 μg of total RNA.
Add the following components to a nuclease-free microcentrifuge tube.
| Component | Volume |
| Total RNA, or miRNA | 50 ng-5 μg |
Or
|
1 µl
1 µl
2 pmol |
| RT-PCR Grade Water | To 12 µl |
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, [email protected]
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