nRT-ROSET, Recombinant M-MLV

 9,334,800 16,816,800

RTase, Recombinant Reverse transcriptase enzyme is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. The enzyme is purified from E. coli expressing a portion of the gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 8 kb. HiPure-Anchored-Oligo (dT)18 primer is provided in the kit for higher yield and full-length cDNA synthesis.

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Description

Kit content of nRT-ROSET Recombinant 

Component 50 Reactions100 Reactions
RTase, Recombinant Reverse transcriptase, RNase H-(200U/μl)50 µl100 µl
5 x RT-Buffer (specialized for RTase)200 µl400 µl
HiPure-Anchored-Oligo (dT)18

Primer (0.5 μg/μl)

50 µl100 µl
HiPure-Random hexamer primer

(0.1 μg/μl)

50 µl100 µl
HiPure-dNTP mix (10 mM, 2.5 mM each)100 µl200 µl
HunteRNase-P, Recombinant (50 U/μl)

(just provided in RT-ROSET Kit)

50 µl100 µl
RT-PCR Grade Water1.5 ml1.5 ml

Storage

nRT-ROSET Recombinant:  Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Intended Use of nRT-ROSET Recombinant 

Notice that, nRT-ROSET and RT-ROSET Kits are intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

nRT-ROSET and RT-ROSET Kits are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Equipment & Reagents to Be Supplied by User

  • Pipets and pipet tips
  • Microcentrifuge tube
  • Thermal cycler
  • Mineral oil (for thermal cyclers without a heated lid)
  • Primers (If want to synthesis a specific kind of cDNA not total cDNA)

Synthesize First-Strand cDNA

A 20-μL reaction volume can be used for 50 ng–5 μg of total RNA.

Add the following components to a nuclease-free microcentrifuge tube.

Component Volume
Total RNA, or miRNA50 ng-5 μg
  • HiPure-Anchored-Oligo (dT)18 primer (0.5 μg/μl)
  • HiPure-Random hexamer primer (0.1 μg/μl)

Or

  • Gene Specific primer
1 µl

1 µl

 

2 pmol

RT-PCR Grade WaterTo 12 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

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Additional information

Size

100 reactions, 50 reactions

Specification

FeaturesSpecifications
EnzymeReverse transcriptase
Master mixNo
Reaction typecDNA synthesis
SampleRNA
Single / MultiplexSingle

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Protocol

none!

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Resources

MSDS nRT-ROSET Kit

Hand Book nRT-ROSET Kit

Quick Protocol EN  FA

Video Protocol Download

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