MiRJia PB Kit,(with RBC Lysis Buffer,1X)

 24,401,520 42,265,080

MiRJia PB Kit, (with RBC Lysis Buffer,1X) uses silica technology and is formulated for rapid and convenient isolation of up to 50 µg miRNA and total RNA (≥18 nucleotides) from whole blood and PBMC. Supplied RBC lysis buffer is sufficient for Up to 3 ml blood. However, the Kit is capable of processing up to 10 ml blood. This Kit provides the possibility of miRNArich fraction isolation. This product contains phenol.

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Description

Kit content

Component50 preps100 preps
RNSol Reagent50 ml100 ml
TWB1 (concentrate)17 ml35 ml
TWB2 (concentrate)15 ml27.5 ml
Nuclease free RBC Lysis Buffer

Just supplied in MiRJia PB Kit with RBC Lysis Buffer

2.5 L5 L
Nuclease-free Water5 ml10 ml
Spin Column50100
Collection Tube50100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol Reagent and nuclease free RBC Lysis buffer should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended use

The MiRJia PB Kit provides the procedures necessary for purifying total RNA from peripheral blood mononuclear cell. Notice that, MiRJia PB Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

MiRJia PB Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Procedure

The MiRJia PB Kit is designed for isolating all forms of RNA molecules from up to 1×107 PBMCs. Fresh or frozen samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of Ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure miRNA and total RNA is finally eluted in nuclease free water. Isolated RNA (miRNA or total RNA) is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to be supplied by user

  • Absolute Ethanol
  • 1.5 ml RNase free microcentrifuge tubes
  • Pipets and RNase free pipet tips
  • Benchtop refrigerated microcentrifuge
  • Vortex
  • TissueLyser/ Mortar and pestle/ Homogenizer
  • Syringe and needles

Applications

The isolated RNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PC
  • Northern, dot blot analysis

Recommended starting material:

To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined RNSol and chloroform amount are written in Table 2.

Table 2. Appropriate sample size and amount of RNSol, Chloroform and Ethanol amount

Whole bloodRNSol AmountChloroform AmountEthanol Amount
1 ml to 3 ml500 µl300 µl400 µl
3 ml to 5 ml700 µl400 µl400 µl
5 ml to 10 ml1 ml500 µl400 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

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Additional information

Size

100 prep, 50 prep

Specification

FeaturesSpecifications
Elution Volume30-100µl
TechnologySilica Technology
Main Sample Type·         PBMC

·         WBC

·         Whole blood

ProcessingManual
Purification of Total RNA, Mirna, Poly A+ Mi-RNA, DNA Or ProteinYes
Sample AmountUp to 107 cells
Operation Time Per ReactionLess Than 60 Min
Typical Yield100 µg
Average PurityA260/A280= 1.9-2.3
Size of RNA PurifiedTotal RNA
EnzymeNo

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Protocol

none!

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Resources

MSDS MiRJia PB kit, with RBC

Hand Book MiRJia PB kit, with RBC

Quick Protocol EN  FA

Video Protocol Download

This post is also available in: Persian