MiRJia PB Kit,(with RBC Lysis Buffer,1X)

Description

Kit content

Component	50 preps	100 preps
RNSol H Reagent	50 ml	100 ml
TWB1 (concentrate)	16 ml	2 x 16 ml
TWB2 (concentrate)	15 ml	2 x 15 ml
Nuclease-free RBC Lysis buffer (prepared just in MiRJia PB Kit, with RBC Lysis Buffer)	2.5 L	5 L
Nuclease-free Water	5 ml	10 ml
HiPure DR Column	50	100
Collection Tube	50	100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol Reagent and nuclease free RBC Lysis buffer should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

MiRJia PB Kit (NO RBC Lysis Buffer) provides the procedures necessary for purifying total RNA from peripheral blood mononuclear cell. Notice that, MiRJia PB Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

MiRJia PB Kit (NO RBC Lysis Buffer) is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Description

MiRJia PB Kit Provides a time-saving, reliable and meticulous method for total RNA isolation (including miRNA) from peripheral blood mononuclear cell. MiRJia PB Kit is based on spin column technology for isolation of concentrated, highly purified and intact total RNA, which is suitable to be used for a variety of downstream applications such as Northern blot experiments, Real-time PCR, RNA Sequencing, Microarray etc.

Procedure

The MiRJia PB Kit is designed for isolating all forms of RNA molecules from up to 1×107 PBMCs. Fresh or frozen samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure miRNA and total RNA is finally eluted in nuclease-free water. Isolated RNA (miRNA or total RNA) is ready to use in downstream applications. It has A260/A280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

• Absolute ethanol
• 1.5 ml RNase free microcentrifuge tubes
• RNase free pipets and pipet tips
• Benchtop refrigerated microcentrifuge (while RNSol H Reagent is used)
• Vortex
• TissueLyser/ Mortar and pestle/ Homogenizer
• Syringe and needles

Applications

The isolated RNA and miRNA can be used in many downstream applications:

• RNA Sequencing
• cDNA synthesis
• Poly A+ RNA selection
• Microarrays
• RT-PCR and real-time RT-PCR
• Northern, dot blot analysis

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

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