Description
Kit content of Hy-Fidelity Pfu PCR set
| Component | 25 Reactions | 100 Reactions | 500 Reactions |
| Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl) | 25 unit (10 µl) | 250 unit (100 µl) | 500 unit (200 µl) |
| PCR Buffer, 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase | 100 µl | 400 µl | 2 x 1 ml |
| HiPure-dNTP mix, 2.5 mM (0.675 mM each) | 200 µl | 800 µl | 4 x 1 ml |
| MgSO4, 50mM, Molecular Biology Grade | 100 µl | 400 µl | 2 ml |
| Water for Molecular Biology, Sterile, Filtered | 1.5 ml | 1.5 ml | 2 x1.5 ml |
Storage
Hy-Fidelity Pfu PCR set: Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.
Intended Use of Hy-Fidelity Pfu PCR set
Notice that, these products are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before
starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com
Quality Control
All ROJETechnologies products are tested against predetermined experiments on a lotto-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com.
Table 2: Prepare PCR Reactions, Hy-Fidelity Pfu PCR Set
| Component | Volume |
| Template | Variable |
| Forward primer (10 µM) | 1 µl |
| Reverse primer (10 µM) | 1 µl |
| Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl) | 1 µl |
| PCR Buffer, 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase | 4 µl |
| HiPure-dNTPs mix, 2.5 mM | 5-8 µl |
| Water for Molecular Biology | Up to 50 µl |
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, Technicalsupport@Rojetechnologies.com
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