Hy-Fidelity Pfu PCR set, (Ready-to-use)

 14,944,000 29,888,000

Hy-Fidelity Pfu PCR set provides a complete kit format comprising, 5t-Pfu DNA Polymerase/ Hy-Fidelity Pfu Polymerase, unique PCR Buffer, HiPure-dNTP Mix and 50 mM molecular biology grade MgSO4. Pfu DNA polymerase is an engineered version of pfu DNA polymerase with enhanced yield and higher fidelity. It possesses a proofreading 3’-5’ exonuclease activity.

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Description

Kit content of Hy-Fidelity Pfu PCR set

Component25 Reactions100 Reactions500 Reactions
Hy-Fidelity Pfu Polymerase,
Recombinant, (2.5U/μl)
25 unit
(10 µl)
250 unit
(100 µl)
500 unit
(200 µl)
PCR Buffer, 10X, with MgSO4,
Optimized for Hy-Fidelity Pfu
Polymerase
100 µl400 µl2 x 1 ml
HiPure-dNTP mix, 2.5 mM
(0.675 mM each)
200 µl800 µl4 x 1 ml
MgSO4, 50mM, Molecular
Biology Grade
100 µl400 µl2 ml
Water for Molecular Biology,
Sterile, Filtered
1.5 ml1.5 ml2 x1.5 ml

Storage

Hy-Fidelity Pfu PCR set: Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Intended Use of Hy-Fidelity Pfu PCR set

Notice that, these products are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before
starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

All ROJETechnologies products are tested against predetermined experiments on a lotto-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com.

Table 2: Prepare PCR Reactions, Hy-Fidelity Pfu PCR Set

ComponentVolume
Template Variable
Forward primer (10 µM)1 µl
Reverse primer (10 µM) 1 µl
Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl)1 µl
PCR Buffer, 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase4 µl
HiPure-dNTPs mix, 2.5 mM 5-8 µl
Water for Molecular BiologyUp to 50 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

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Additional information

Size

250 unit, 500 unit

Specification

This post is also available in: Persian

Protocol

none!

This post is also available in: Persian

Resources

MSDS Hy-Fidility pfu PCR

Hand Book Hy-Fidility pfu PCR

Quick Protocol EN  FA

Video Protocol Download

This post is also available in: Persian