Hy-Fidelity Pfu PCR set, (Ready-to-use)

 14,944,000 29,888,000

Hy-Fidelity Pfu PCR set provides a complete kit format comprising, 5t-Pfu DNA Polymerase/ Hy-Fidelity Pfu Polymerase, unique PCR Buffer, HiPure-dNTP Mix and 50 mM molecular biology grade MgSO4. Pfu DNA polymerase is an engineered version of pfu DNA polymerase with enhanced yield and higher fidelity. It possesses a proofreading 3’-5’ exonuclease activity.

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Description

Kit content of Hy-Fidelity Pfu PCR set

Component 250 Reactions 500 Reactions
Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl) 100 µl 200 µl
PCR Buffer 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase 400 µl 800 µl

Storage

Hy-Fidelity Pfu PCR set: Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Intended Use of Hy-Fidelity Pfu PCR set

Notice that, these products are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before
starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

All ROJETechnologies products are tested against predetermined experiments on a lotto-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com.

Table 2: Prepare PCR Reactions, Hy-Fidelity Pfu PCR Set

Component Volume
Template Variable
Forward primer (10 µM) 1 µl
Reverse primer (10 µM) 1 µl
Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl) 1 µl
PCR Buffer, 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase 4 µl
HiPure-dNTPs mix, 2.5 mM 5-8 µl
Water for Molecular Biology Up to 50 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

250 unit, 500 unit

Specification

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Protocol

none!

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Resources

MSDS Hy-Fidility pfu PCR

Hand Book Hy-Fidility pfu PCR

Quick Protocol EN  FA

Video Protocol Download

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