DNJia AmnioPure Kit content
|Component||50 preps||100 preps|
|ALB||13 ml||26 ml|
|BWB1 (concentrate)||15 ml||30 ml|
|BWB2 (concentrate)||15 ml||30 ml|
|RRB||5 ml||5 ml|
|RJ-Protease||1 ml||2 x 1 ml|
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.
DNJia AmnioPure Kit provides the components and procedures necessary for purifying genomic DNA from amniocytes. Notice that, DNJia AmnioPure Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies.
DNJia AmnioPure Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies
DNJia AmnioPure Kit is designed for isolating DNA from amniocytes. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and ALB. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of Ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure genomic DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/A 280 ratios of 1.7-2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.
Equipment & Reagents to be supplied by user
- Ethanol (96-100%)
- Preparation of phosphate buffered saline (PBS)
- Pipets and pipet tips
- 5 ml Microtube
- Centrifuge and Micro centrifuge
- Dry Heat Block/ Water Bath
The isolated DNA can be used in many downstream applications:
- Different kinds of PCRs, including Long-range PCR
- Restriction digestion
- Southern blotting
Recommended Starting Material
The size of recommended starting material to use with determined lysis volume is listed here.
Table 2: Recommended starting material and Lysis Buffer amount
|Sample||Size of Starting material||ALB Amount|
|Under 18 weeks||5-6 ml of amniotic fluid||250 µl|
|Above 18 weeks||2-3 ml of amniotic fluid|
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, Technicalsupport@Rojetechnologies.com
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