DNJia AmnioPure Kit

 15,284,400 23,460,000

DNJia AmnioPure Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic DNA isolation from amniocytes. The procedure is based on spin column technology, which takes less than 45 minutes. DNJia AmnioPure Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. The isolated DNA is ready to use in downstream applications such as PCR analysis and restriction endonuclease digestions.

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Description

DNJia AmnioPure Kit content

Component 50 preps 100 preps
ALB 13 ml 26 ml
BWB1 (concentrate) 15 ml 30 ml
BWB2 (concentrate) 15 ml 30 ml
RRB 5 ml 5 ml
RJ-Protease 1 ml 2 x 1 ml
Spin Columns 50 100
Collection Tube 50 100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

DNJia AmnioPure Kit provides the components and procedures necessary for purifying genomic DNA from amniocytes. Notice that, DNJia AmnioPure Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study DNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies.

Quality Control

DNJia AmnioPure Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies

Procedure

DNJia AmnioPure Kit is designed for isolating DNA from amniocytes. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and ALB.  Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of Ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure genomic DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/A 280 ratios of 1.7-2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

Equipment & Reagents to be supplied by user

  • Ethanol (96-100%)
  • Preparation of phosphate buffered saline (PBS)
  • Pipets and pipet tips
  • 5 ml Microtube
  • Vortex
  • Centrifuge and Micro centrifuge
  • Dry Heat Block/ Water Bath

Applications

The isolated DNA can be used in many downstream applications:

  • Different kinds of PCRs, including Long-range PCR
  • Sequencing
  • Restriction digestion
  • Southern blotting
  • Cloning

Recommended Starting Material

The size of recommended starting material to use with determined lysis volume is listed here.

Table 2: Recommended starting material and Lysis Buffer amount

Sample Size of Starting material ALB Amount
Under 18 weeks 5-6 ml of amniotic fluid 250 µl
Above 18 weeks 2-3 ml of amniotic fluid

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

100 reactions, 50 reactions

Specification

Features Specifications
Elution volume 25-30 µl
Technology Silica technology
Main sample type Amniotic fluid
Processing  Manual
Sample amount Up to 6 ml of amniotic fluid
Operation time per reaction Less than 45 Min
Typical yield Varies
Average purity A260/A280= 1.7-2.0
Size of DNA purified ≈ 50 Kb
Enzyme RJ-Protease

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Protocol

none!

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Resources

MSDS DNJia AmnioPure Kit

Hand Book DNJia AmnioPure Kit

Quick Protocol EN  FA

Video Protocol Download

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