DNJia AmnioPure Kit

 16,500,000 30,500,000

DNJia AmnioPure Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic DNA isolation from amniotic fluid. The procedure is based on spin column technology, which takes less than 20 minutes. DNJia AmnioPure Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. Typical yields of genomic DNA vary, depending on cell density of samples. The isolated DNA is ready to use in downstream applications such as PCR analysis and restriction endonuclease digestions.

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Description

DNJia AmnioPure Kit content

Component 50 preps 100 preps
GLB 10 ml 20 ml
BWB1 (concentrate) 16 ml 2 X 16 mll
BWB2 (concentrate) 12 ml 2 X 12 ml
RRB 5 ml 10 ml
RJ-Protease 1.25 ml 2 x 1.25 ml
HiPure DR Column 50 100
Collection Tube 50 100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

DNJia AmnioPure Kit provides the components and procedures necessary for purifying genomic DNA from amniotic fluid. Notice that, DNJia AmnioPure Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.

 Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

DNJia AmnioPure Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

 Description

DNJia AmnioPure Kit provides a rapid, careful, convenient and phenol-free method for high quality genomic DNA isolation from amniotic fluid. The procedure is based on spin column technology, which takes less than 20 minutes. DNJia AmnioPure Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. Typical yields of genomic DNA vary, depending on cell density of samples. The isolated DNA is ready to use in downstream applications such as PCR analysis and restriction endonuclease digestions.

Procedure

DNJia AmnioPure Kit is designed for isolating DNA from 5-6 ml amniotic fluid. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and GLB. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/A 280 ratios of 1.7-2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

 Equipment & Reagents to Be Supplied by User

  • Ethanol (96-100%)
  • Pipets and pipet tips
  • 1.5 ml Microtube
  • Vortex
  • Centrifuge
  • Micro centrifuge
  • Dry Heat Block/ Water Bath

Applications

The isolated DNA can be used in many downstream applications:

  • Different kinds of PCRs, including Long-range PCR
  • Sequencing
  • Restriction digestion
  • Southern blotting

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

100 reactions, 50 reactions

Specification

Features Specifications
Elution volume 25-30 µl
Technology Silica technology
Main sample type Amniotic fluid
Processing  Manual
Sample amount Up to 6 ml of amniotic fluid
Operation time per reaction Less than 45 Min
Typical yield Varies
Average purity A260/A280= 1.7-2.0
Size of DNA purified ≈ 50 Kb
Enzyme RJ-Protease

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Protocol

none!

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Resources

MSDS DNJia AmnioPure Kit

Hand Book DNJia AmnioPure Kit

Quick Protocol EN  FA

Video Protocol Download

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