DNall Kit

 10,707,600 19,274,400

DNall Kit provides a rapid, careful, convenient method for high quality genomic DNA isolation from various sample types including blood, buffy coat, body fluid, buccal cells, animal tissue, serum and plasma, fixed tissue, cultured cells and bacteria. This kit is based on spin column technology for isolation of concentrated, highly purified, intact genomic DNA which is suitable to use for variety of downstream processes such as PCR analysis, restriction endonuclease digestions, Southern blot, genotyping and etc.

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Description

DNall Kit content

Component50 preps100 preps
TLB9 ml18 ml
GLB12.5 ml25 ml
BWB1 (concentrate)17 ml35 ml
BWB2 (concentrate)15 ml32 ml
RRB10 ml20 ml
RJ-Protease1 ml2 X 1 ml
Spin Columns50100
Collection Tube100200

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

DNall Kit provides the components and procedures necessary for purifying genomic DNA from blood, buffy coat, body fluid, buccal cells, animal tissue, serum and plasma, fixed tissue, cultured cells and bacteria. Notice that, DNall Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study DNA experiments guideline, before starting their work.

Procedure

DNall Kit is designed for isolating DNA from various sample types. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and ROJE specific lysis buffer. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of chaotropic salts and Ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure genomic DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/ A 280   ratios of 1.7–2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

DNall Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Equipment & Reagents to be supplied by user

  • Ethanol (96-100%)
  • Pipets and pipet tips
  • 5 ml Microtube
  • Vortex
  • Centrifuge
  • Micro centrifuge
  • Dry Heat Block/ Water Bath

Applications

The isolated DNA can be used in many downstream applications:

  • Different kinds of PCRs, including Long-range PCR
  • Sequencing
  • Restriction digestion
  • Southern blotting
  • Cloning

Recommended Starting Material

To reach optimized results it is better to follow as listed here. The size of recommended material is written in Table 2.

Table 2: Appropriate size of starting material

SampleSize of Starting Material
Blood*Up to 250 µl
Buffy coatUp to 200 µl
Animal tissue All tissue kind expect spleen: up to 25 mg

(spleen up to 10 mg)

Bacteria cells2.5 x 10 8 to 2 x 109
Cultured cell≤5 x 106 cells

*White Blood Cell counts are extremely variable from a person to person and therefore, for optimum results its recommended to determine cell counts for choosing the best protocol for purification. For healthy individuals, white blood cell counts normally from 4-10 million per 1ml blood; however, depending on disease state, cell counts may be higher or lower than the normal range.

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

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Additional information

Size

100 reactions, 50 reactions

Specification

FeaturesSpecifications
Elution volume50-200µl
TechnologySilica technology
Main sample typeFresh whole blood/ buffy coat/ body fluids/ serum and plasma/ animal tissue/ cultured cells/ bacteria
ProcessingManual
Sample amount·   10-25 mg of tissue

·   Up to 2×109 bacteria cells

·   Up to 5 X 106 cultured cells

·   Up to 200µl blood, buffy coat, body fluid

Operation time per reactionLess Than 30 Min (for whole blood, buffy coat, serum and plasma)

Less than 2 h (for animal tissue and bacteria)

Typical yield· Varies depending on sample type
Average purityA260/A280= 1.7-2.0
Size of DNA purified≈ 50 Kb

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Protocol

none!

This post is also available in: Persian

Resources

MSDS DNall Kit

Hand Book DNall Kit

Quick Protocol EN  FA

Video Protocol Download

This post is also available in: Persian