DNall Kit

 15,000,000 28,000,000

DNall Kit provides a rapid, careful, convenient method for high quality genomic DNA isolation from various sample types including blood, buffy coat, body fluid, buccal cells, animal tissue, serum and plasma, fixed tissue, cultured cells and bacteria. This kit is based on spin column technology for isolation of concentrated, highly purified, intact genomic DNA which is suitable to use for variety of downstream processes such as PCR analysis, restriction endonuclease digestions, Southern blot, genotyping and etc.

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Description

DNall Kit content

Component 50 preps 100 preps
TLB 9 ml 18 ml
GLB 12.5 ml 25 ml
BWB1 (concentrate) 16 ml 2 x 16 ml
BWB2 (concentrate) 16 ml 2 x 16 ml
RRB 10 ml 20 ml
RJ-Protease 1 ml 2 X 1 ml
HiPure OD Column 50 100
Collection Tube 100 200

Storage

 Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

 DNall Kit provides the components and procedures necessary for purifying genomic DNA from blood, buffy coat, body fluid, buccal cells, animal tissue, serum and plasma, fixed tissue, cultured cells and bacteria. Notice that, DNall Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.

Guarantee & Warranty

 ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

 Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

DNall Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

 DNall Kit provides a rapid, careful, convenient method for high quality genomic DNA isolation from various sample types including blood, buffy coat, body fluid, buccal cells, animal tissue, serum and plasma, fixed tissue, cultured cells and bacteria. This kit is based on spin column technology for isolation of concentrated, highly purified, intact genomic DNA which is suitable to use for variety of downstream processes such as PCR analysis, restriction endonuclease digestions, Southern blot, genotyping and etc.

 Procedure

 DNall Kit is designed for isolating DNA from various sample types. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and ROJE specific lysis buffer. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of chaotropic salts and ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure genomic DNA is finally eluted in rehydration buffer. Isolated 7 DNA is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.7– 2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

 Equipment & Reagents to Be Supplied by User

  • Ethanol (96-100%)
  • Pipets and pipet tips
  • 1.5 ml Microtube
  • Vortex
  • Centrifuge
  • Micro centrifuge
  • Dry Heat Block/ Water Bath

Applications

 The isolated DNA can be used in many downstream applications:

  • Different kinds of PCRs, including Long-range PCR
  • Sequencing
  • Restriction digestion
  • Southern blotting
  • Cloning

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

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Additional information

Size

100 reactions, 50 reactions

Specification

Features Specifications
Elution volume 50-200µl
Technology Silica technology
Main sample type Fresh whole blood/ buffy coat/ body fluids/ serum and plasma/ animal tissue/ cultured cells/ bacteria
Processing Manual
Sample amount ·   10-25 mg of tissue

·   Up to 2×109 bacteria cells

·   Up to 5 X 106 cultured cells

·   Up to 200µl blood, buffy coat, body fluid

Operation time per reaction Less Than 20 Min (for whole blood, buffy coat, serum and plasma)

Less than 2 h (for animal tissue and bacteria)

Typical yield · Varies depending on sample type
Average purity A260/A280= 1.7-2.0
Size of DNA purified ≈ 50 Kb

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Protocol

none!

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Resources

MSDS DNall Kit

Hand Book DNall Kit

Quick Protocol EN  FA

Video Protocol Download

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