|Components||48 reactions||96 reactions|
|B-5, B-51 Master Mix, 2X||288 µl||576 µl|
|B-5 Primer Mix||65 µl||130 µl|
|B-51 Primer Mix||65 µl||130 µl|
Shipment condition of HLA B51B5 Diagnostic Kit is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at -20 °C. Do not perform more than 3 freezing/thawing cycles to the B-5, B-51 Master Mix, 2X vial as this could reduce the assays sensitivity and change results. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.
Intended Use of HLA B51B5 Diagnostic Kit
HLA B5 and B51 Diagnostic Kit is an in vitro nucleic acid amplification test for rapid group-specific detection of HLA B-5 and B-51 alleles of human major histocompatibility complex in the biological materials (whole blood and oropharyngeal swabs). Notice that, Assays for the molecular typing of HLA B-5 and B-51 is based on polymerase chain reaction (PCR). We recommend all users to study PCR experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com
HLA B5 and B51 Diagnostic Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, QC sheet and the results of all experiments are accessible by addressing Cat and Lot number on our website at rojetechnologies.com
Primer pairs are designed for typing HLA B-5 and B-51 alleles. HLA locus-specific amplification is performed in a thermal cycler using the amplification primer mix, template DNA, and the B-5, B-51 Master Mix, 2X. After successful amplification, the genomic DNA target sequence is present in a detectable concentration. Each reaction contains an internal control primer pair, which amplifies a conserved region of a housekeeping gene in every PCR reaction mix showing if the PCR reaction had taken place and to exclude results based on inhibitors, non-sufficient DNA and displaced primers from other wells. After the PCR process, the amplified DNA fragments are size-separated by agarose gel electrophoresis and exposure to ultraviolet light, documented by photography and interpreted. Interpretation of PCR-SSP results is based on the presence or absence of specific PCR product(s). The relative sizes of the specific PCR product(s) may be helpful in the interpretation of the results.
Equipment & Reagents to Be Supplied by User
- Thermal Cycler
- Pipets and pipet tips
- Microwave oven / Heating Equipment
- Agarose gel electrophoresis system
- PCR tray rack
- Mineral oil (for thermal cyclers without a heated lid)
- Population studies
- Disease associations
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, Technicalsupport@Rojetechnologies.com
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