MiRJia Kit

 20,283,120 36,447,840

MiRJia Kit uses silica technology and is formulated for rapid and convenient isolation of up to 200 µg total RNA from animal cells and tissue. This Kit provides the possibility of miRNArich fraction isolation. This product contains phenol.

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Description

Kit content

Component50 preps100 preps
RNSol Reagent 50 ml100 ml
TWB1 (concentrate)17 ml35 ml
TWB2 (concentrate)15 ml27.5 ml
Nuclease-free Water5 ml10 ml
Spin Column50100
Collection Tube50100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol reagent should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

The MiRJia Kit provides the procedures necessary for purifying total RNA from various sample type. Notice that, MiRJia Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

MiRJia Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Procedure

The MiRJia Kit is designed for isolating all form of RNA molecules from 0.5-100 mg tissue samples or up to 1×107 cells. Fresh or frozen tissue samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of Ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure miRNA and total RNA is finally eluted in nuclease free water. Isolated RNA (miRNA or total RNA) is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

  • Absolute Ethanol
  • 1.5 ml RNase free microcentrifuge tubes
  • Pipets and RNase free pipet tips
  • Benchtop refrigerated microcentrifuge
  • Vortex
  • TissueLyser/ Mortar and pestle/ Homogenizer
  • Syringe and needles

Applications

The isolated RNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PC
  • Northern, dot blot analysis

Recommended starting material:

To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined RNSol and chloroform amount are written in Table 2.

Table 2. Appropriate sample size and amount of RNSol, Chloroform and Ethanol amount

Sample SizeRNSol AmountChloroform Amount
10 mg animal tissue,

1 × 106 animal cells

100 µl100 µl
20 mg animal tissue

2 × 106 animal cells

200 µl100 µl
30 mg animal tissue

3 × 106 animal cells

300 µl200 µl
40 mg animal tissue

4 × 106 animal cells

400 µl200 µl
50 mg animal tissue

5 × 106 animal cells

1-3 ml whole Blood

500 µl300 µl
60 mg animal tissue

6 × 106 animal cells

600 µl300 µl
70 mg animal tissue

7 × 106 animal cells

3-5 ml whole Blood

700 µl400 µl
80 mg animal tissue

8 × 106 animal cells

800 µl400 µl
90 mg animal tissue

9 × 106 animal cells

900 µl500 µl
100 mg animal tissue

107 animal cells

5-10 ml whole Blood

1ml500 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

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Additional information

Size

100 prep, 50 prep

Specification

FeaturesSpecifications
Elution Volume50-100µl
TechnologySilica Technology
Main Sample TypeAnimal Cells and Tissue/ Bacteria Cells
ProcessingManual
Purification of Total RNA, Mirna, Poly A+ Mi-RNA, DNA Or ProteinYes
Sample Amount0.5-100 Mg
Operation Time Per ReactionLess Than 45 Min
Typical Yield100 µg
Average PurityA260/A280= 1.9-2.3
Size of RNA PurifiedTotal RNA
EnzymeNo

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Protocol

none!

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Resources

MSDS MiRJia Kit

Hand Book MiRJia Kit

Quick Protocol EN  FA

Video Protocol Download

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