RNJia Lipid Kit

 20,283,600 36,447,600

RNJia Lipid Kit provides a timesaving, reliable and meticulous method for RNA isolation from adipose animal tissues and cells. RNJia Lipid Kit is based on HiPure DR Column technology for isolation of concentrated, highly purified and intact total RNA, which is suitable to be used for a variety of downstream applications such as northern blot experiments, real-time PCR, RNA sequencing, microarray etc.

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Description

Kit content

Component 50 preps 100 preps
RNSol Reagent 50 ml 100 ml
TWB1 (concentrate) 16 ml 2 x 16 ml
TWB2 (concentrate) 15 ml 2 x 15 ml
Nuclease-free Water 5 ml 10 ml
Spin Column 50 100
Collection Tube 50 100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on kit box.

Intended Use

The RNJia Lipid Kit provides the procedures necessary for purifying total RNA from various sample type. Notice that, RNJia Lipid Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

RNJia Lipid Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies

Description

RNJia Lipid Kit provides a timesaving, reliable and meticulous method for RNA isolation from adipose animal tissues and cells. RNJia Lipid Kit is based on HiPure DR Column technology for isolation of concentrated, highly purified and intact total RNA, which is suitable to be used for a variety of downstream applications such as northern blot experiments, real-time PCR, RNA sequencing, microarray etc.

Procedure

The RNJia Lipid Kit is designed for isolating all form of RNA molecules from 0.5-100 mg tissue samples. Fresh or frozen tissue samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure RNA is finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream applications. It has A260/A280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

  • Absolute ethanol
  • 1.5 ml RNase free microcentrifuge tubes
  • RNase free pipets and pipet tips
  • Benchtop refrigerated microcentrifuge (while RNSol H Reagent is used)
  • Vortex
  • Thermoblock or water bath
  • TissueLyser/ Mortar and pestle (for animal tissue sample)
  • Syringe and needles
  • Homogenizer

Applications

The isolated RNA and miRNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PCR
  • Northern, dot blot analysis

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

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Additional information

Size

100 prep, 50 prep

Specification

Features Specifications
Elution Volume 30-100µl
Technology Silica Technology
Main Sample Type Animal adipose tissue
Processing Manual
Sample Amount 0.5-100 Mg
Purification of Total RNA, Mirna, Poly A+ Mi-RNA, DNA Or Protein Yes
Operation Time Per Reaction Less than 45 min
Typical Yield 2-20 µg
Average Purity A260/A280= 1.9-2.3
Size of RNA Purified RNA ≥200 nt
Enzyme No

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Protocol

none!

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Resources

MSDS RNJia Lipid Kit

Hand Book RNJia Lipid Kit

Quick Protocol EN  FA

Video Protocol Download

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