RNJia Lipid Kit

 20,283,600 36,447,600

RNJia Lipid Kit Provides a timesaving, reliable and meticulous method for RNA isolation from adipose animal tissues and cells. RNJia Lipid Kit is based on spin column technology for isolation of concentrated, highly purified and intact total RNA, which is suitable to be used for a variety of downstream applications such as northern blot experiments, real-time PCR, RNA sequencing, microarray etc.

Category: SKU: N/A


Kit content

Component50 preps100 preps
RNSol Reagent50 ml100 ml
TWB1 (concentrate)17 ml35 ml
TWB2 (concentrate)15 ml27.5 ml
Nuclease-free Water5 ml10 ml
Spin Column 50100
Collection Tube 50100


Shipment condition is checked by ROJETechnologies. After arrival, RNSol should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on kit box.

Intended Use

The RNJia Lipid Kit provides the procedures necessary for purifying total RNA from various sample type. Notice that, RNJia Lipid Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

RNJia Lipid Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure
consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies


The RNJia Lipid Kit is designed for isolating all form of RNA molecules from 0.5-100 mg tissue samples. Fresh or frozen tissue samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of Ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure RNA is finally eluted in nuclease free water. Isolated RNA is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to be supplied by user

  • Absolute Ethanol
  • Chloroform
  • 1.5 ml RNase free microcentrifuge tubes
  • Pipets and RNase free pipet tips
  • Benchtop microcentrifuge
  • Vortex
  • TissueLyser/ Mortar and pestle/ Homogenizer
  • Syringe and needles


The isolated RNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PC
  • Northern, dot blot analysis

Recommended starting material:

To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined RNSol and chloroform amount are written in Table 2.
Table 2. Appropriate sample size and amount of RNSol and Chloroform amount for Animal Adipose tissue

Sample SizeRNSol AmountChloroform Amount
10 mgAnimal Adipose tissue100 µl100 µl
20 mg Animal Adipose tissue200 µl100 µl
30 mg Animal Adipose tissue300 µl100 µl
40 mg Animal Adipose tissue400 µl100 µl
50 mg Animal Adipose tissue500 µl200 µl
60 mg Animal Adipose tissue600 µl200 µl
70 mg Animal Adipose tissue700 µl200 µl
80 mg Animal Adipose tissue800 µl200 µl
90 mg Animal Adipose tissue900 µl200 µl
100 mg Animal Adipose tissue1ml200 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

This post is also available in: Persian

Additional information


100 prep, 50 prep


Elution Volume30-100µl
TechnologySilica Technology
Main Sample TypeAnimal adipose tissue
Sample Amount0.5-100 Mg
Purification of Total RNA, Mirna, Poly A+ Mi-RNA, DNA Or ProteinYes
Operation Time Per ReactionLess than 45 min
Typical Yield2-20 µg
Average PurityA260/A280= 1.9-2.3
Size of RNA PurifiedRNA ≥200 nt

This post is also available in: Persian



This post is also available in: Persian


MSDS RNJia Lipid Kit

Hand Book RNJia Lipid Kit

Quick Protocol EN  FA

Video Protocol Download

This post is also available in: Persian


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