RNJia Lipid Kit

 20,283,600 36,447,600

RNJia Lipid Kit Provides a timesaving, reliable and meticulous method for RNA isolation from adipose animal tissues and cells. RNJia Lipid Kit is based on spin column technology for isolation of concentrated, highly purified and intact total RNA, which is suitable to be used for a variety of downstream applications such as northern blot experiments, real-time PCR, RNA sequencing, microarray etc.

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Description

Kit content

Component 50 preps 100 preps
RNSol Reagent 50 ml 100 ml
TWB1 (concentrate) 17 ml 35 ml
TWB2 (concentrate) 15 ml 27.5 ml
Nuclease-free Water 5 ml 10 ml
Spin Column 50 100
Collection Tube 50 100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on kit box.

Intended Use

The RNJia Lipid Kit provides the procedures necessary for purifying total RNA from various sample type. Notice that, RNJia Lipid Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

RNJia Lipid Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure
consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies

Procedure

The RNJia Lipid Kit is designed for isolating all form of RNA molecules from 0.5-100 mg tissue samples. Fresh or frozen tissue samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of Ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure RNA is finally eluted in nuclease free water. Isolated RNA is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to be supplied by user

  • Absolute Ethanol
  • Chloroform
  • 1.5 ml RNase free microcentrifuge tubes
  • Pipets and RNase free pipet tips
  • Benchtop microcentrifuge
  • Vortex
  • TissueLyser/ Mortar and pestle/ Homogenizer
  • Syringe and needles

Applications

The isolated RNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PC
  • Northern, dot blot analysis

Recommended starting material:

To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined RNSol and chloroform amount are written in Table 2.
Table 2. Appropriate sample size and amount of RNSol and Chloroform amount for Animal Adipose tissue

Sample Size RNSol Amount Chloroform Amount
10 mgAnimal Adipose tissue 100 µl 100 µl
20 mg Animal Adipose tissue 200 µl 100 µl
30 mg Animal Adipose tissue 300 µl 100 µl
40 mg Animal Adipose tissue 400 µl 100 µl
50 mg Animal Adipose tissue 500 µl 200 µl
60 mg Animal Adipose tissue 600 µl 200 µl
70 mg Animal Adipose tissue 700 µl 200 µl
80 mg Animal Adipose tissue 800 µl 200 µl
90 mg Animal Adipose tissue 900 µl 200 µl
100 mg Animal Adipose tissue 1ml 200 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

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Additional information

Size

100 prep, 50 prep

Specification

Features Specifications
Elution Volume 30-100µl
Technology Silica Technology
Main Sample Type Animal adipose tissue
Processing Manual
Sample Amount 0.5-100 Mg
Purification of Total RNA, Mirna, Poly A+ Mi-RNA, DNA Or Protein Yes
Operation Time Per Reaction Less than 45 min
Typical Yield 2-20 µg
Average Purity A260/A280= 1.9-2.3
Size of RNA Purified RNA ≥200 nt
Enzyme No

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Protocol

none!

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Resources

MSDS RNJia Lipid Kit

Hand Book RNJia Lipid Kit

Quick Protocol EN  FA

Video Protocol Download

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