RNJia Virus Kit

Description

 Kit content:

Component	100 preps
BFC	56 ml
BWB1 (concentrate)	22 ml
BWB2 (concentrate)	16.5 ml
ERR	10 ml
Carrier RNA	620  μg
HiPure DR Column	100
Collection Tube	200

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. Carrier RNA also can be store at room temperature, however after preparation; it should be divided into conveniently sized aliquots and stored at -20 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

RNJia Virus Kit provides the components and procedures necessary for purifying viral RNA from cell-free samples such as body fluid, serum, plasma etc. RNJia Virus Kit is intended for molecular biology applications. This kit has IVD certificate to be used for SARS-COV-2 isolation. We recommend all users to study RNA experiments guideline, before starting their work.

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

RNJia Virus Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

The RNJia Virus Kit is designed for rapid and efficient purification of high-quality viral RNA from various human and animal liquid samples such as body fluid, plasma, serum etc. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, which takes less than 25 minutes. RNJia Virus Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorite for laboratories with many isolations in a day. The purified RNA is free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated RNA can be directly used in PCR, qPCR or other nucleic acid-based assays.

Procedure

RNJia Virus Kit is designed for isolating RNA from body fluid, serum, plasma etc. Lysis is achieved by incubation of the sample in BFC buffer. Appropriate conditions for RNA binding to the silica membrane is achieved by the addition of ethanol to the lysate. Then, RNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure viral RNA is finally eluted in rehydration buffer. Isolated RNA is ready to use in downstream applications.

Equipment & Reagents to Be Supplied by User

• Ethanol (96-100%)
• Sterile, RNase-free pipets and pipet tips
• 1.5 ml Microtube
• Vortex 6
• Centrifuge and Micro centrifuge
• Dry Heat Block/ Water Bath

Applications

The isolated RNA can be used in many downstream applications:

• Different kinds of PCRs
• Restriction enzyme digestion
• Viral genotyping
• Viral detection
• Viral load monitoring

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

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