RNJia Phenol Free PB Kit, (NO RBC Lysis Buffer)

Description

Kit content

Component	50 preps	100 preps
RLB	30 ml		60 ml
TWB1 (concentrate)	16 ml		2 x 16 ml
TWB2 (concentrate)	15 ml		2 x 15 ml
Nuclease-free RBC Lysis buffer Just included in RNJia Phenol-free PB Kit, (With RBC Lysis Buffer)	375 ml		750 ml
Nuclease-free Water	5 ml		10 ml
HiPure DR Column	50		100
Collection Tube	50		100

 

Storage

Shipment condition is checked by ROJETechnologies. After arrival, Nuclease-free RBC Lysis buffer should be kept at 2-8 °C and all other reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

RNJia Phenol-free PB Kit provides the components and procedures necessary for purifying RNA and miRNA from PBMC, whole blood and WBC. Notice that, RNJia Phenol-free PB Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

RNJia Phenol-free PB Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

RNJia phenol-free PB Kit provides a time-saving, reliable and meticulous method for RNA isolation (including miRNA) from peripheral blood mononuclear cell, WBC and whole blood. RNJia phenol-free PB Kit is based on spin column technology for isolation of concentrated, highly purified and intact RNA, which is suitable to be used for a variety of downstream applications such as Northern blot experiments, Real-time PCR, RNA sequencing, Microarray etc.

Procedure

The RNJia phenol-free PB Kit is designed for isolating all forms of RNA molecules from up to 1×107 WBCs. Fresh or frozen samples should be lysed and homogenized. Then, RNA binding to the silica membrane is achieved selectively, by addition of ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure RNA is finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream applications. It has A260/A280 ratios of 1.8–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

• Absolute ethanol
• 1.5 ml RNase free microcentrifuge tubes
• RNase free pipets and pipet tips
• Vortex
• Thermoblock or water bath
• TissueLyser/ Mortar and pestle (for animal tissue sample)
• Syringe and needles
• Homogenizer

Applications

The isolated RNA and miRNA can be used in many downstream applications:

• RNA Sequencing
• cDNA synthesis
• Poly A+ RNA selection
• Microarrays
• RT-PCR and real-time RT-PCR
• Northern, dot blot analysis

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

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