RNJia Phenol Free PB Kit, (NO RBC Lysis Buffer)

 16,412,400 28,828,800

RNJia phenol-free PB Kit Provides a time-saving, reliable and meticulous method for RNA isolation (including miRNA) from peripheral blood mononuclear cell, WBC and whole blood. RNJia phenol-free PB Kit is based on spin column technology for isolation of concentrated, highly purified and intact RNA, which is suitable to be used for a variety of downstream applications such as Northern blot experiments, Real-time PCR, RNA Sequencing, Microarray and etc.

Clear
Category: SKU: N/A

Description

Kit content

Component50 preps100 preps
RLB30 ml60 ml
TWB1 (concentrate)17 ml35 ml
TWB2 (concentrate)15 ml27.5 ml
Nuclease-free RBC Lysis buffer

Just included in RNJia Phenol-free PB Kit,

(With RBC Lysis Buffer)

375 ml750 ml
Nuclease-free Water5 ml6 ml
Spin Column50100
Collection Tube50100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, Nuclease-free RBC Lysis buffer should be kept at 2-8 °C and all other reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use of RNJia Phenol Free PB Kit 

The RNJia phenol-free PB Kit provides the procedures necessary for purifying RNA from peripheral blood mononuclear cell, WBC and whole blood. Notice that, RNJia phenol-free PB Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

RNJia phenol-free PB Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Procedure

The RNJia phenol-free PB Kit is designed for isolating all forms of RNA molecules from up to 1×107 WBCs. Fresh or frozen samples should be lysed and homogenized. Then, RNA binding to the silica membrane is achieved selectively, by the addition of Ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure RNA is finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream applications. It has A260/A280 ratios of 1.8–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

  • Absolute Ethanol
  • 1.5 ml RNase free microcentrifuge tubes
  • Pipets and RNase free pipet tips
  • Benchtop microcentrifuge
  • Vortex
  • Homogenizer/ Syringe and needles

 

Applications of RNJia Phenol Free PB Kit

The isolated RNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PCR
  • Northern, dot blot analysis

Recommended starting material:

To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined RLB amount are written in Table 2.

Table 2. Appropriate sample size and amount of RLB.

Whole blood (ml)WBCs NumberRLB Amount (µl)
Up to 0.5Up to 2 x 106350
0.5 to 1.52 x 106 to 1 x 107600

Technical Assistance 

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

This post is also available in: Persian

Additional information

Size

100 prep, 50 prep

Specification

FeaturesSpecifications
Elution Volume30-50 µl
TechnologySilica Technology
Main Sample Type·         PBMC

·         Whole blood

·         WBC

ProcessingManual
Purification of Total RNA, Mirna, Poly A+ Mi-RNA, DNA Or ProteinTotal RNA
Sample AmountUp to 1.5 ml whole blood (1 X 107 cells)
Operation Time Per Reaction45- 75 Min
Typical Yield4.5-20 µg
Average PurityA260/A280= 1.8-2.3
Size of RNA PurifiedRNA ≥ 200 bp
EnzymeNo

This post is also available in: Persian

Protocol

none!

This post is also available in: Persian

Resources

MSDS RNJia Phenol-Free PB kit, No RBC

Hand Book RNJia Phenol-Free PB kit, No RBC

Quick Protocol EN  FA

Video Protocol Download

This post is also available in: Persian