Description
PCR-Pure Kit content
Component | 100 preps |
RPB | 2 x 28 ml |
GWB | 2 x 10 ml |
RSB | 10 ml |
HiPure DR column | 100 |
Collection tube | 100 |
Storage
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.
Intended Use
Description
PCR-Pure Kit provides a rapid, precise and convenient method for efficient nucleic acid purification. The procedure is based on spin column technology, which takes less than 15 minutes. Special buffers provided with kit are enhanced for wellorganized DNA recovery and contaminants removal. Due to presence of high concentration of salts, DNA adsorbs to the silica membrane and contaminants pass through the column. Finally, DNA is solved at prepared ROJE rehydration buffer. Typical yield, depends on density of starting material. The isolated nucleic acid is ready to use in downstream applications such as restriction endonuclease digestions
PCR-Pure Kit provides the components and procedures necessary for purifying PCR product. Notice, Kits are intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies
Quality Control
PCR-Pure Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies
Equipment & Reagents to be supplied by user
- Isopropanol/ Absolute Ethanol
- Pipets and pipet tips
- 5 ml Microcentrifuge tube
- Vortex
- Centrifuge
- Microcentrifuge
- Dry Heat block / Water Bath
Applications
The isolated DNA can be used in many downstream applications:
- Sequencing
- Restriction digestion
- Cloning
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, Technicalsupport@Rojetechnologies.com
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