RNJia PB Kit,(NO RBC Lysis Buffer)

 20,283,600 36,448,800

RNJia PB Kit Provides a time-saving, reliable and meticulous method for total RNA isolation (including miRNA) from peripheral blood mononuclear cell. RNJia PB Kit is based on spin column technology for isolation of concentrated, highly purified and intact total RNA, which is suitable to be used for a variety of downstream applications such as Northern blot experiments, Real-time PCR, RNA Sequencing, Microarray etc.

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Description

Kit content

Component 50 preps 100 preps
RNSol H Reagent 50 ml 100 ml
TWB1 (concentrate) 16 ml 2 x 16 ml
TWB2 (concentrate) 15 ml 2 x 15 ml
Nuclease-free RBC Lysis buffer (prepared just
in RNJia PB Kit, with RBC Lysis Buffer)
2.5 L 5 L
Nuclease-free Water 5 ml 10 ml
HiPure DR Column 50 100
Collection Tube 50 100

 

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol H Reagent and Nuclease-free RBC Lysis Buffer should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

RNJia PB Kit provides the components and procedures necessary for purifying RNA and miRNA from PBMC, whole blood and WBC. Notice that, RNJia PB Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

RNJia PB Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

RNJia PB Kit Provides a time-saving, reliable and meticulous method for total RNA isolation (including miRNA) from peripheral blood mononuclear cell. RNJia PB Kit is based on spin column technology for isolation of concentrated, highly purified and intact total RNA, which is suitable to be used for a variety of downstream applications such as Northern blot experiments, Real-time PCR, RNA Sequencing, Microarray etc.

Procedure

The RNJia PB Kit is designed for isolating all forms of RNA molecules from up to 1 ×107 PBMCs. Fresh or frozen samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure miRNA and total RNA is finally eluted in nuclease-free water. Isolated RNA (miRNA or total RNA) is ready to use in downstream applications. It has A260/A280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

  • Absolute ethanol
  • 1.5 ml RNase free microcentrifuge tubes
  • RNase free pipets and pipet tips
  • Benchtop refrigerated microcentrifuge (while RNSol H Reagent is used)
  • Vortex 6
  • TissueLyser/ Mortar and pestle/ Homogenizer
  • Syringe and needles

Applications

The isolated RNA and miRNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PCR
  • Northern, dot blot analysis

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

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Additional information

Size

100 prep, 50 prep

Specification

Features Specifications
Elution Volume 30-100µl
Technology Silica Technology
Main Sample Type ·         PBMC

·         Whole blood

·         WBC

Processing Manual
Purification of Total RNA, Mirna, Poly A+ Mi-RNA, DNA Or Protein Yes
Sample Amount Up to 107 cells
Operation Time Per Reaction Less Than 60 Min
Typical Yield 100 µg
Average Purity A260/A280= 1.9-2.3
Size of RNA Purified Total RNA
Enzyme No

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Protocol

none!

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Resources

MSDS RNJia PB kit, No RBC

Hand Book RNJia PB kit, No RBC

Quick Protocol EN  FA

Video Protocol Download

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