Description
Kit content
| Component | 50 preps | 100 preps |
| RLB | 50 ml | 100 ml |
| TWB1 (concentrate) | 17 ml | 35 ml |
| TWB2 (concentrate) | 15 ml | 27.5 ml |
| Nuclease-free Water | 5 ml | 6 ml |
| Spin Column | 50 | 100 |
| Collection Tube | 50 | 100 |
Storage
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature (15-25°C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box. All enzymes should be kept at -20 °C for long term storage and for routine use 2-8 °C is suitable.
Intended Use of RNJia Kit
The RNJia Kit provides the components and procedures necessary for purifying RNA from cultured cells, animal tissue and bacteria cells. Notice that, RNJia is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies
Quality Control
RNJia is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies
Procedure
The RNJia is designed for isolating all RNA molecules larger than 200 nucleotides from 0.5-30 mg tissue samples or up to 1×107 animal cells. Fresh or frozen tissue samples should be lysed and homogenized. Then, RNA binding to the silica membrane is achieved selectively, by the addition of Ethanol to the lysate. Contaminants removed by two specific kinds of washing buffers. Pure RNA is finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream amplification. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.
Equipment & Reagents to Be Supplied by User
- Absolute Ethanol
- 1.5 ml RNase free microcentrifuge tubes
- RNase free Pipets and pipet tips
- Benchtop microcentrifuge
- Vortex
- Thermoblock or water bath
- TissueLyser/ Mortar and pestle (for animal tissue sample)
- Syringe and needles
- Homogenizer
Applications
The isolated RNA can be used in many downstream applications:
- RNA Sequencing
- cDNA synthesis
- Poly A+ RNA selection
- Microarrays
- RT-PCR and real-time RT-PCR
- Northern, dot blot analysis
Recommended starting material:
To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined lysis volumes are written in Table 2.
Table 2. Appropriate sample size and amount of RLB
| Sample Type | Sample Size | Amount of RLB |
| Animal Cells | ≤5 X106 | 350 µl |
| Animal Cells | 5 X 106 To 1 X 107 | 600 µl |
| Animal Not Fibrous Tissues | 0.5-10 Mg | 350 µl |
| Animal Not Fibrous Tissues | 10-30 Mg | 600 µl |
| Animal Fibrous Tissues | 0.5-30 Mg | 300 µl |
| Bacteria Cells | ≤5 X108 | 500 µl |
| Bacteria Cells | 5 X 108 – 1 X 109 | 800 µl |
| Bacteria Cells | 1 X 109 – 2 X 109 | 1000 µl |
| Whole blood | Up to 0.5 ml | 350 µl |
| Whole blood | 0.5 to 1.5 ml | 600 µl |
| WBCs Number | Up to 2 x 106 | 350 µl |
| WBCs Number | 2 x 106 to 1 x 107 | 600 µl |
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, Technicalsupport@Rojetechnologies.com
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