RNJia Kit

 16,412,400 28,828,800

RNJia Kit Provides a time-saving, reliable and meticulous method for RNA isolation from various sample types including animal tissues and cells and bacteria. This kit can be used for RNA isolation from bacteria, lipid and fibrous tissue, however we suggested to use RNJia Bacteria Kit, RNJia Lipid Kit and RNJia Fibrous kits, which are specialized for RNA isolation from bacteria, lipid and fibrous tissues to reach optimum results, however RNJia is capable of isolating RNA from these sample types if suitable enzymes are purchased. RNJia kit is based on spin column technology for isolation of concentrated, highly purified and intact RNA, without use of phenol and chloroform, which is suitable to use for variety of downstream processes such as Northern blot experiments, Real-time PCR, RNA Sequencing, Microarray and etc.

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Description

Kit content

Component50 preps100 preps
RLB50 ml100 ml
TWB1 (concentrate)17 ml35 ml
TWB2 (concentrate)15 ml27.5 ml
Nuclease-free Water5 ml6 ml
Spin Column50100
Collection Tube50100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature (15-25°C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box. All enzymes should be kept at -20 °C for long term storage and for routine use 2-8 °C is suitable.

Intended Use of RNJia Kit

The RNJia Kit provides the components and procedures necessary for purifying RNA from cultured cells, animal tissue and bacteria cells. Notice that, RNJia is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.co .

Quality Control

RNJia is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com .

Procedure

The RNJia is designed for isolating all RNA molecules larger than 200 nucleotides from 0.5-30 mg tissue samples or up to 1×107 animal cells. Fresh or frozen tissue samples should be lysed and homogenized. Then, RNA binding to the silica membrane is achieved selectively, by the addition of Ethanol to the lysate. Contaminants removed by two specific kinds of washing buffers. Pure RNA is finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream amplification. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

  • Absolute Ethanol
  • 1.5 ml RNase free microcentrifuge tubes
  • RNase free Pipets and pipet tips
  • Benchtop microcentrifuge
  • Vortex
  • Thermoblock or water bath
  • TissueLyser/ Mortar and pestle (for animal tissue sample)
  • Syringe and needles
  • Homogenizer

Applications

The isolated RNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PCR
  • Northern, dot blot analysis

Recommended starting material:

To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined lysis volumes are written in Table 2.

Table 2. Appropriate sample size and amount of RLB

Sample TypeSample SizeAmount of RLB
Animal Cells≤5 X106350 µl
Animal Cells5 X 106 To 1 X 107600 µl
Animal Not Fibrous Tissues0.5-10 Mg350 µl
Animal Not Fibrous Tissues10-30 Mg600 µl
Animal Fibrous Tissues0.5-30 Mg300 µl
Bacteria Cells≤5 X108500 µl
Bacteria Cells5 X 108 – 1 X 109800 µl
Bacteria Cells1 X 109 – 2 X 1091000 µl
Whole bloodUp to 0.5 ml350 µl
Whole blood0.5 to 1.5 ml600 µl
WBCs NumberUp to 2 x 106350 µl
WBCs Number2 x 106 to 1 x 107600 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

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Additional information

Size

100 prep, 50 prep

Specification

FeaturesSpecifications
Elution Volume (µl)30-100 µl
TechnologySilica technology
Main Sample Typeanimal cells and tissue /Bacteria cells
ProcessingManual
Sample AmountVaries

 

Operation Time Per Reaction (Min)Less than 30min
Typical Yield (µg)60 µg
Average Purity (A260/A280, A260/A230)A260/A280= 1.95-2.3
Size of RNA Purified≥200 nucleotides
EnzymeOnly for fibrous tissue and bacteria

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Protocol

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Resources

MSDS RNJia Kit

Hand Book RNJia Kit

Quick Protocol EN  FA

Video Protocol Download

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