RNSol H Reagent

Description

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol H reagent should be kept dry, at 2-8 ̊ C. When storage condition is as directed, RNSol H reagent is stable until expiration date, as indicated on the kit box. All enzymes should be kept at -20 °C for long term storage and for routine use 2-8 °C is suitable.

Intended Use

The RNSol H reagent provides the procedures necessary for isolating RNA from cultured cells, animal tissue, whole blood and bacteria cells. Notice that, RNSol H is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies

 Quality Control

RNSol H reagent is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies

Procedure

The RNSol H reagent is designed for isolating all form of RNA molecules from 0.5-100 mg tissue samples or up to 1×10⁷ animal cells and up to 2 x 10⁹ bacteria cells. Fresh or frozen tissue samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA selectively will be isolated by the addition of chloroform. Contaminants removed by 75% Ethanol. Pure RNA is finally eluted in nuclease free water. Isolated RNA is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to Be Supplied by User

• Isopropanol
• Nuclease free Water
• Chloroform
• 1.5 ml RNase free microcentrifuge tubes
• Pipets and RNase free pipet tips
• RNase free microcentrifuge tube
• Benchtop refrigerated microcentrifuge
• Vortex
• Heat block / water bath
• TissueLyser/ Homogenizer/ Mortar and pestle

Applications

The isolated DNA can be used in many downstream applications:

• RNA Sequencing
• cDNA synthesis
• Poly A+ RNA selection
• Microarrays
• RT-PCR and real-time RT-PC
• Northern, dot blot analysis

Recommended starting material:

To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined RNSol H and chloroform amount are written in Table 2.

Table 2. Appropriate sample size and amount of RNSol H, chloroform and isopropanol amount

Sample Size	RNSol H Amount	Chloroform Amount	Isopropanol
50-100 mg animal tissue	1 ml	200 µl	500 µl
1 × 105 – 107 Cells grown in monolayer	400 µl	80 µl	200 µl
5-10 × 106 Cells grown in suspension	750 µl	150 µl	375 µl
≤5 × 108 bacteria cells	400 µl	200 µl	400 µl
5 × 108 – 109 bacteria cells	500 µl	300 µl	500 µl
0.5 – 1 ml whole blood	500 µl	100 µl	250 µl
1 – 5 ml whole blood	1000 µl	200 µl	400 µl
5 – 10 ml whole blood	1200 µl	200 µl	500 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]