Description
Storage
Shipment condition is checked by ROJETechnologies. After arrival, RNSol H Reagent should be kept dry, at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.
Intended Use
RNSol H Reagent is used to purify RNA and miRNA from animal tissue and cell, PBMC, whole blood, WBC and bacteria. Notice that, RNSol H Reagent is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.
Guarantee & Warranty
ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.
Quality Control
RNSol H Reagent is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.
Description
RNSol H reagent provides a reliable and meticulous method for total RNA isolation from various sample types including animal tissues and cells. This reagent can be used for RNA isolation from bacteria and fibrosis tissue, such as muscle, heart etc. RNSol H is suitable for isolation of concentrated, highly purified and intact RNA, which is suitable to be used for a variety of downstream applications. As in process phenol and chloroform are applied, it is recommended to use RNJia Kit for situations in which the purified RNA will be used in sensitive downstream applications.
Procedure
The RNSol H reagent is designed for isolating all form of RNA molecules from 0.5- 100 mg tissue samples or up to 1×107 animal cells and up to 2 x 109 bacteria. Fresh or frozen tissue samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA selectively will be isolated by the addition of chloroform. Contaminants removed by 75% ethanol. Pure RNA is finally eluted in nuclease free water. Isolated RNA is ready to use in downstream applications. It has A260/A280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.
Equipment & Reagents to Be Supplied by User
- Isopropanol
- Nuclease free Water
- Chloroform
- 1.5 ml RNase free microcentrifuge tubes
- Pipets and RNase free pipet tips
- RNase free microcentrifuge tube
- Benchtop refrigerated microcentrifuge
- Vortex • Heat block / water bath
- TissueLyser/ Homogenizer/ Mortar and pestle
Applications
The isolated RNA and miRNA can be used in many downstream applications:
- RNA Sequencing
- cDNA synthesis
- Poly A+ RNA selection
- Microarrays
- RT-PCR and real-time RT-PCR
- Northern, dot blot analysis
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, [email protected]
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