RT-ROSET, Recombinant M-MLV, (with RNase inhibitor)

 11,325,600 20,385,600

RT-ROSET kit contains all necessary components for conversion of total RNA or mRNA to the single stranded cDNA. The RTmix solutions contains, RTbuffer, 1mM dNTP mixture, 8mM MgC12,01igo d(t)16, Random hexamer and stabilizer. MixZyme contains thermostable H-minus MMLV, RNase Inhibitor and stabilizer.

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Category: SKU: N/A

Description

Kit content of RT-ROSET Recombinant

Component 50
reactions 
100
reactions
RTase, Recombinant Reverse
transcriptase, RNase H-(200U/μl)
50 µl 100 µl
5 x RT-Buffer (specialized for RTase) 200 µl 400 µ
HiPure-Anchored-Oligo (dT)18
Primer (0.5 μg/μl)
50 µl 100 µl
HiPure-Random hexamer primer
(0.1 μg/μl)
50 µl 100 µl
HiPure-dNTP mix (10 mM, 2.5 mM each) 100 µl 200 µl
HunteRNase-P, Recombinant (50 U/μl)
(just provided in RT-ROSET Kit)
50 µl 100 µl
RT-PCR Grade Water 1.5 ml 1.5 ml

Storage

RT-ROSET Recombinant: Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components
are stable until expiration date, as indicated on the kit box.

Intended Use of RT-ROSET Recombinant

Notice that, nRT-ROSET and RT-ROSET Kits are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

nRT-ROSET and RT-ROSET Kits are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management
system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Equipment & Reagents to Be Supplied by User

  • Pipets and pipet tips
  • Microcentrifuge tube
  • Thermal cycler
  • Mineral oil (for thermal cyclers without a heated lid)
  • Primers (If want to synthesis a specific kind of cDNA not total cDNA)

Synthesize First-Strand cDNA

A 20-μL reaction volume can be used for 50 ng–5 μg of total RNA.
Add the following components to a nuclease-free microcentrifuge tube.

Table 2: Appropriate size of starting material

Component Volume
Total RNA, or miRNA 50 ng-5 μg
 HiPure-Anchored-Oligo (dT)18 primer (0.5 μg/μl) 1 µl
HiPure-Random hexamer primer (0.1 μg/μl) 1 µl
Or
 Gene Specific primer
2 pmol
RT-PCR Grade Water To 12 µl

General Tip

1. Always wear latex or vinyl gloves while handling reagents and RNA samples to prevent RNase contamination from the surface of the skin or from dusty laboratory mobilizations. These materials are generally RNase-free and do not require pretreatment to inactivate RNase.

2. Treat surfaces of benches with commercially available RNase inactivating agents such as RNZO (Order by cat No BU983026 and BU983027).

3. Treat non-disposable glassware and plasticware before use, to ensure that it is RNase-free. Thoroughly rinse plasticware with 0.1N NaOH/1mM EDTA, and RNase-free water.

4. Use disposable RNase-free plasticware to decrease the possibility of sample contamination.

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

100 reactions, 50 reactions

Specification

Features Specifications
Enzyme Reverse transcriptase
Master mix No
Reaction type cDNA synthesis
Sample RNA
Single / Multiplex Single

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Protocol

none!

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Resources

MSDS RT-ROSET Kit

Hand Book RT-ROSET Kit

Quick Protocol EN  FA

Video Protocol Download

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