Description
Kit content
| Component | 50 preps |
| RNSol Reagent | 50 ml |
| TWB1 (concentrate) | 16 ml |
| TWB2 (concentrate) | 15 ml |
| Nuclease-free Water | 5 ml |
| Spin Column | 50 |
| Collection Tube | 50 |
Storage
Intended Use
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com
Quality Control
RNJia Lipid Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies
Description
RNJia Lipid Kit provides a timesaving, reliable and meticulous method for RNA isolation from adipose animal tissues and cells. RNJia Lipid Kit is based on HiPure DR Column technology for isolation of concentrated, highly purified and intact total RNA, which is suitable to be used for a variety of downstream applications such as northern blot experiments, real-time PCR, RNA sequencing, microarray etc.
Procedure
The RNJia Lipid Kit is designed for isolating all form of RNA molecules from 0.5-100 mg tissue samples. Fresh or frozen tissue samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure RNA is finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream applications. It has A260/A280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.
Equipment & Reagents to Be Supplied by User
- Absolute ethanol
- 1.5 ml RNase free microcentrifuge tubes
- RNase free pipets and pipet tips
- Benchtop refrigerated microcentrifuge (while RNSol H Reagent is used)
- Vortex
- Thermoblock or water bath
- TissueLyser/ Mortar and pestle (for animal tissue sample)
- Syringe and needles
- Homogenizer
Applications
The isolated RNA and miRNA can be used in many downstream applications:
- RNA Sequencing
- cDNA synthesis
- Poly A+ RNA selection
- Microarrays
- RT-PCR and real-time RT-PCR
- Northern, dot blot analysis
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, [email protected]
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