Description
Kit content
| Component | 50 preps | 100 preps | |
| RLB | 15 ml | 30 ml | |
| TWB1 (concentrate) | 17 ml | 35 ml | |
| TWB2 (concentrate) | 15 ml | 27.5 ml | |
| Nuclease-free Water | 5 ml | 6 ml | |
| RJ-Protease | 1 ml | 2 X 1 ml | |
| Spin Column | 50 | 100 | |
| Collection Tube | 50 | 100 | |
Storage
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature (15-25°C). When storage condition is as directed, all reagents are stable until expiration date, indicated on the kit box. All enzymes should be kept at -20 °C for long term storage and for routine use 2-8 °C is suitable.
Intended Use
The RNJia Fibrous Kit provides the components and procedures necessary for purifying RNA from cultured cells, animal tissue and bacteria cells. However, it is specialized for RNA isolation from fibrous tissue. Notice that, RNJia Fibrous Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies
Quality Control
RNJia Fibrous Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies
Procedure
The RNJia Fibrous Kit is designed for isolating all RNA molecules longer than 200 nucleotides from 0.5-30 mg tissue samples. Fresh or frozen tissue samples should be lysed and homogenized. Then, RNA binding to the silica membrane is achieved selectively, by the addition of chaotropic salts and Ethanol to the lysate. Contaminants removed by two specific kind of washing buffers. Pure RNA is finally eluted in nuclease-free water. Isolated RNA is ready to use in downstream amplification. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.
Equipment & Reagents to Be Supplied by User
- Absolute Ethanol
- 1.5 ml RNase free microcentrifuge tubes
- Pipets and RNase free pipet tips
- Benchtop microcentrifuge
- Vortex
- Heating block
- TissueLyser/ Homogenizer/ Mortar and pestle
- Syringe and needles
Applications
The isolated RNA can be used in many downstream applications:
- RNA Sequencing
- cDNA synthesis
- Poly A+ RNA selection
- Microarrays
- RT-PCR and real-time RT-PC
- Northern, dot blot analysis
Recommended starting material:
To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined lysis volumes are written in Table 2.
Table 2. Appropriate sample size and amount of RLB
| Sample Type | Sample Size | Amount Of Rlb | |
| Animal Fibrous Tissues | 0.5-30 mg | 300 µl | |
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, Technicalsupport@Rojetechnologies.com
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