HLA B27 Diagnostic Kit, SSP-PCR assay

 5,016,000 9,028,800

HLA B-27 Diagnostic Kit is designed to determine HLA B-27 alleles based on the Polymerase Chain Reaction method using sequence specific primer (PCR-SSP assay), which enables amplification of defined DNA sequences. The principle of the SSP assay is to generate an amplicon only when the sequence of a primer is perfectly complementary to the target sequence of a DNA sample. On the other hand, noncomplementary primer do not bind to the DNA and then no amplification takes place. Evaluation of the result is performed by agarose gel electrophoresis. In the electric field the amplicons separate according to their size. If no amplification takes place, no specific band is seen. This kit is tested for 1094 individuals and has shown 100% concordance compared to sequencing, showing 100% specificity and sensitivity in this population size.

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Description

Kit content

Components48 reactions96 reactions
B-27 Master Mix, 2X  123 μl247 μl
B-27 Primer Mix312 μl624 μl

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at -20 °C. Do not perform more than 3 freezing/thawing cycles to the B-27 Master Mix, 2X vial as this could reduce the assays sensitivity and change results. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use of HLA B27 Diagnostic Kit

HLA B27 Diagnostic Kit is an in vitro nucleic acid amplification test for rapid group-specific detection of HLA B-27 alleles of human major histocompatibility complex in the biological materials (whole blood and oropharyngeal swabs). Notice that, Assay for the molecular typing of HLA B-27 is based on polymerase chain reaction (PCR). We recommend all users to study PCR experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

HLA B27 Diagnostic Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, QC sheet and the results of all experiments are accessible by addressing Cat and Lot number on our website at rojetechnologies.com

Procedure

Primer pairs are designed for typing HLA B-27 alleles. HLA locus-specific amplification is performed in a thermal cycler using the amplification primer mix, template DNA, and the B-27 Master Mix, 2X. After successful amplification, the genomic DNA target sequence is present in a detectable concentration. In all reaction  an internal control primer pair exist, which amplifies a conserved region of a housekeeping gene in every PCR reaction mix showing if the PCR reaction had taken place and to exclude results based on inhibitors, non-sufficient DNA and displaced primers from other wells. After the PCR process, the amplified DNA fragments are size-separated by agarose gel electrophoresis and exposure to ultraviolet light, documented by photography and interpreted. Interpretation of PCR-SSP results is based on the presence or absence of specific PCR product(s). The relative sizes of the specific PCR product(s) may be helpful in the interpretation of the results.

Equipment & Reagents to be supplied by user

  • Thermal Cycler
  • Pipets and pipet tips
  • Microwave oven / Heating Equipment
  • Agarose gel electrophoresis system
  • PCR tray rack
  • Mineral oil (for thermal cyclers without a heated lid)

Applications

  • Population studies
  • Disease associations
  • Pharmacogenomics

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

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Additional information

Size

48 reaction, 96 reaction

Specification

FeaturesSpecifications
EnzymeRecombinant Taq Polymerase
Reaction typePCR Amplification
SamplegDNA, cDNA
Single / MultiplexMultiplex

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Protocol

none!

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Resources

MSDS Download

Hand Book HLA B-27

Quick Protocol EN  FA

Video Protocol Download

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