ROMAX Plus 2X PCR Master- Mix

 2,400,000

ROMAX Plus, 2X which is a highly enhanced version of ROMAX 2X provides a complete ingredient needed for PCR include; Taq DNA polymerase, PCR buffer, dNTPs, except DNA template and primers. By reducing pipetting steps, ROMAX Plus, 2X help to reduce contamination. The enzyme consists of a single polypeptide with molecular weight of approximately 94 k Da. It has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity. This enzyme is suitable for routine amplification.

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Description

Description

ROMAX Plus, 2X which is a highly enhanced version of ROMAX 2X provides a complete ingredient needed for PCR include; Taq DNA polymerase, PCR buffer, dNTPs, except DNA template and primers. By reducing pipetting steps, ROMAX Plus, 2X help to reduce contamination. The enzyme consists of a single polypeptide with molecular weight of approximately 94 k Da. It has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity. This enzyme is suitable for routine amplification.

 Application

  • High throughput PCR.
  • Routine PCR
  • Generation of PCR products for TA. Cloning
  • RT-PCR

Protocol

  1. Thawing ROMAX Plus, 2X; then gently invert and briefly centrifuge.
  2. Place PCR tube on ice and add following component to each tube.
ROMAX Plus, 2X 25 µl
Forward primer (10 µM) 1 µl
Reverse primer (10 µM) 1 µl
Template Variable
Water for Molecular Biology Up to 50 µl
Total volume 50 µl
  1. Spin down the PCR tube and run the PCR program.
Step Temp Time Cycle
Initial denaturation 95 ̊C 1-3 min 1
Denaturation 95 ̊C 30 s  

30

 

Annealing Tm 30 s
Extension 72 1 min/Kb
Final
Extension
72 5-15 min 1

 

Certificate of analysis

  • Endodeoxyribonuclease Assay

No detectable degradation of DNA was observed after incubation of 1 µg of pUC19 DNA with 25 µL of ROMAX Plus, 2X in 50 µL of reaction mixture for 4 hours at 37°C and at 70°C.

  • Exodeoxyribonuclease Assay No detectable degradation of DNA was observed after incubation of 1 μg of lambda DNA/HindIII fragments with 25 μL of ROMAX Plus (2X) in 50 μL of reaction mixture for 4 hours at 37°C and at 70°C.
  • Ribonuclease Assay

No contaminating RNase activity was detected after incubation of 1 µg of [3H]-RNA with 25 µL ROMAX Plus, 2X in 50 µL of reaction mixture for 4 hours at 37°C and at 70°C.

  • Functional Assay

ROMAX Plus, 2X was tested for amplification of 1,110 bp single copy gene from human genomic DNA.

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support team at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

50 reactions

Specification

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Protocol

none!

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Resources

MSDS Download

Hand Book Download

Quick Protocol EN  FA

Video Protocol Download

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