Hy-Taq Polymerase, Recombinant

Description

Hy-Taq Polymerase, Recombinant Kit content

Component	250 Reactions	500 Reactions
Hy-Taq Polymerase, Recombinant (5U/μl)	(50 µl)	(100 µl)
PCR Buffer, 10X, with Mg++, optimized for Hy-Taq Polymerase	250 µl	500 µl

 

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

 Intended Use

Notice that, these products are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

 Guarantee & Warranty

 ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

All ROJETechnologies products are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

Recombinant Hy-Taq polymerase is purified from E. coli expressing cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with molecular weight of approximately 94kDa. It has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity. This enzyme is suitable for routine amplification.

Prepare PCR Reactions

It is recommended to prepare PCR reactions as indicated in Table2.
Table 2. Prepare PCR Reactions, Si-Taq Polymerase

Component	Volume
Template	Variable
Forward primer (10 µM)	1 µl
Reverse primer (10 µM)	1 µl
Hy-Taq Polymerase, Recombinant (5U/μl)	0.5 – 1 µl
PCR Buffer, 10X, with Mg++, optimized for Hy-Taq polymerase	5 µl
HiPure-dNTP mix, 2.5 mM	8 µl
Water for Molecular Biology, Sterile, Filtered	Up to 50 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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