Hy-Taq Polymerase, Recombinant

 1,428,000 2,572,800

Hy-Taq polymerase is purified from E. coli expressing cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with molecular weight of approximately 94kDa. It has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity. This enzyme is suitable for routine amplification.

Clear

Description

Hy-Taq Polymerase, Recombinant Kit content

Component250 Reactions500 Reactions
Hy-Taq Polymerase, Recombinant (5U/μl)250 unit

(50 µl)

500 unit

(100 µl)

PCR Buffer, 10X, with Mg++, optimized for Hy-Taq Polymerase1.25 ml2 x 1.25 ml

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Intended Use of Hy-Taq Polymerase

Notice that, these products are intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

All ROJETechnologies products are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Prepare PCR Reactions

It is recommended to prepare PCR reactions as indicated in Table2.
Table 2. Prepare PCR Reactions, Si-Taq Polymerase

Component Volume
TemplateVariable
Forward primer (10 µM)1 µl
Reverse primer (10 µM)1 µl
Hy-Taq Polymerase, Recombinant (5U/μl)0.5 – 1 µl
PCR Buffer, 10X, with Mg++, optimized for Hy-Taq polymerase5 µl
HiPure-dNTP mix, 2.5 mM8 µl
Water for Molecular Biology, Sterile, FilteredUp to 50 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

This post is also available in: Persian

Additional information

Size

250 unit, 500 unit

Specification

This post is also available in: Persian

Protocol

This post is also available in: Persian

Resources

MSDS Hy-Taq Polymerase, Recombinant

Hand Book Hy-Taq Polymerase, Recombinant

Quick Protocol EN  FA

Video Protocol Download

This post is also available in: Persian