Introduction; Immune response profile in Hesperidin treated mice suffering from experimental autoimmune encephalomyelitis.
Immune response profile in Hesperidin treated mice suffering from experimental autoimmune encephalomyelitis: Multiple sclerosis (MS) is the most common central nervous system inflammatory disease, which is due to the reaction of auto-reactive T cells with myelin proteins, leading to physical disorder and paralysis among people suffering the disease. Experimental autoimmune encephalomyelitis (EAE) is used as an animal model of this disease.
Twenty four C57/BL6 female mice, aging 6-8 weeks and weighing 20 gr were used in this project. The mice were divided into 3 groups as follows: 1. Normal group 2. Control group 3.treatment group. For induction of EAE a mixture of Myelin oligodendrocyte Glycoprotein and complete freund’s adjuvant were injected subcutaneously. Mice were also intraperitoneally (i.p.) injected with pertussis toxin. A second identical injection of pertussis toxin was given after 48h. Parallel to EAE induction, the treatment group for 21 days was daily injected i.p 200 mg/kg Hesperidin. On 21 day mice were anesthetized and sacrificed. Percents of FoxP3+ regulatory splenocytes by flow cytometry, levels of IL-4, IL-17 and IFN- using ELISA and splenocytes proliferation assay by Brdu, were all covered to determine the profile of immune response.
Despite increased in the rate of FoxP3+ regulatory T cells and IL-4 secretion, the levels of IFN-, IL-17 and the proliferation of splenocytes all showed remarkable decrease in Hesperidin treated group.
Discussion; Immune response profile in Hesperidin treated mice suffering from experimental autoimmune encephalomyelitis
This study suggests that hesperidin shifted the immune response to Th2.