Kit content of Si-ROJE PCR Set
|Component||250 Reactions||500 Reactions|
|PCR Buffer, 10X, with Mg++,
Optimized for Si-Taq Polymerase
|1.25 ml||2 x 1.25 ml|
|HiPure-dNTP mix, 2.5 mM
(0.675 mM each)
|2 x 1 ml||4 x 1 ml|
|Sor, 6X, DNA Loading Dye,
|0.5 ml||5 x 1 ml|
|Water for Molecular Biology,
|1.5 ml||2×1.5 ml|
Intended Use of Si-ROJE PCR Set
Notice that, these products are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.
Tips for Optimizing PCR Reaction
A final concentration of 2mM MgSO4 is sufficient for most targets amplification. For some targets, more Mg2+ may be required; use the 50 mM MgSO4 stock to test from
2 mM to 4 mM (final concentration) in 0.25 mM increments.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eye-wear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com
All ROJETechnologies products are tested against predetermined experiments on a lotto-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com .
Recommended starting material:
To reach optimized results it is better to follow as listed here. The size of recommended material is written in Table 2.
Table 2. Prepare PCR Reactions, Si-ROJE PCR Set
|Forward primer (10 µM)||1 µl|
|Reverse primer (10 µM)||1 µl|
|Si-Taq Polymerase, Recombinant (5U/μl)||0.5 – 1 µl|
|PCR Buffer, 10X, with Mg++, optimized for Si-Taq polymerase||5 µl|
|HiPure-dNTP mix, 2.5 mM||8 µl|
|Water for Molecular Biology, Sterile, Filtered||Up to 50 µl|
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• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com) Or send your questions to this email address, [email protected]