Si-ROJE PCR Set,(Ready-to-use)

﷼ 1,544,400 – ﷼ 2,934,000

Si-ROJE PCR Set provides a complete kit format comprising, Si-Taq DNA Polymerase/Hy-Taq DNA Polymerase, unique PCR Buffer, HiPure-dNTP Mix and Loading dye. Recombinant Taq polymerase is purified from E. coli expressing cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with molecular weight of approximately 94kDa. It has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity. This enzyme is suitable for routine amplification.

Description

Kit content of Si-ROJE PCR Set

Component	250 Reactions	500 Reactions
Si-Taq Polymerase, Recombinant (5U/μl)	250 unit (50 µl)	500 unit (100 µl)
PCR Buffer, 10X, with Mg++, Optimized for Si-Taq Polymerase	1.25 ml	2 x 1.25 ml
HiPure-dNTP mix, 2.5 mM (0.675 mM each)	2 x 1 ml	4 x 1 ml
Sor, 6X, DNA Loading Dye, Red	0.5 ml	5 x 1 ml
Water for Molecular Biology, Sterile, Filtered	1.5 ml	2×1.5 ml

Storage

Si-ROJE PCR Set: Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Intended Use of Si-ROJE PCR Set

Notice that, these products are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Tips for Optimizing PCR Reaction

A final concentration of 2mM MgSO4 is sufficient for most targets amplification. For some targets, more Mg2+ may be required; use the 50 mM MgSO4 stock to test from
2 mM to 4 mM (final concentration) in 0.25 mM increments.

Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eye-wear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control
All ROJETechnologies products are tested against predetermined experiments on a lotto-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com .

Recommended starting material:

To reach optimized results it is better to follow as listed here. The size of recommended material is written in Table 2.

Table 2. Prepare PCR Reactions, Si-ROJE PCR Set

Component	Volume
Template	Variable
Forward primer (10 µM)	1 µl
Reverse primer (10 µM)	1 µl
Si-Taq Polymerase, Recombinant (5U/μl)	0.5 – 1 µl
PCR Buffer, 10X, with Mg++, optimized for Si-Taq polymerase	5 µl
HiPure-dNTP mix, 2.5 mM	8 µl
Water for Molecular Biology, Sterile, Filtered	Up to 50 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product. Contact our technical support at any time by selecting one of these ways:
• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com) Or send your questions to this email address, [email protected]

Additional information

Size	250 unit, 500 unit

Specification

Protocol

none!

Resources

MSDS Si-ROJE PCR Set

Hand Book Si-ROJE PCR Set

Quick Protocol EN FA

Video Protocol Download

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