ROMAX provides a complete ingredient which is need for PCR include; Taq DNA polymerase, PCR buffer, dNTPs, except DNA template and primers. By reducing pipetting steps, ROMAX help to reduce contamination. The enzyme consists of a single polypeptide with molecular weight of approximately 94 k Da. It has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity. This enzyme is suitable for routine amplification.
• High throughput PCR.
• Routine PCR
• Generation of PCR products for TA. Cloning
1. Thawing ROMAX, (2X), then gently invert and briefly centrifuge.
2. Place PCR tube on ice and add following component to each tube.
|ROMAX 2X||25 µl|
|Forward primer (10 µM)||1 µl|
|Reverse primer (10 µM)||1 µl|
|Water for Molecular Biology||Up to 50 µl|
|Total volume||50 µl|
3. Spin down the PCR tube and run the PCR program.
|Initial denaturation||95 ̊C||1-3 min||1|
|Denaturation||95 ̊C||30 s|| |
Certificate of analysis
- Endodeoxyribonuclease Assay
No detectable degradation of DNA was observed after incubation of 1 µg of pUC19 DNA with 25 µL of PCR ROMAX (2X) in 50 µL of reaction mixture for 4 hours at 37°C and at 70°C.
- Exodeoxyribonuclease Assay
No detectable degradation of DNA was observed after incubation of 1 μg of lambda DNA/HindIII fragments with 25 μL of ROMAX (2X) in 50 μL of reaction mixture for 4 hours at 37°C and at 70°C.
- Ribonuclease Assay
No contaminating RNase activity was detected after incubation of 1 µg of [3H]-RNA with 25 µL ROMAX (2X) in 50 µL of reaction mixture for 4 hours at 37°C and at 70°C.
- Functional Assay
ROMAX (2X) was tested for amplification of 597 bp and 268 bp single copy genes from human genomic DNA.
ROMAX 2X PCR Master Storage
ROMAX 2X PCR Master: Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease and Prime-RNase A at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the inner surface of the kit lid.
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Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
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