ROMAX 2X PCR Master- Mix

Description

Description

ROMAX provides a complete ingredient which is need for PCR include; Taq DNA polymerase, PCR buffer, dNTPs, except DNA template and primers. By reducing pipetting steps, ROMAX help to reduce contamination. The enzyme consists of a single polypeptide with molecular weight of approximately 94 k Da. It has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity. This enzyme is suitable for routine amplification.

Application

• High throughput PCR.

• Routine PCR

• Generation of PCR products for TA. Cloning

• RT-PCR

Protocol

1. Thawing ROMAX, (2X), then gently invert and briefly centrifuge.

2. Place PCR tube on ice and add following component to each tube.

ROMAX 2X	25 µl
Forward primer (10 µM)	1 µl
Reverse primer (10 µM)	1 µl
Template	Variable
Water for Molecular Biology	Up to 50 µl
Total volume	50 µl

 

3. Spin down the PCR tube and run the PCR program.

Step	Temp	Time	Cycle
Initial denaturation	95 ̊C	1-3 min	1
Denaturation	95 ̊C	30 s	30
Annealing	Tm	30 s
Extension	72	1 min/Kb
Final Extension	72	5-15 min	1

 

Certificate of analysis

• Endodeoxyribonuclease Assay

No detectable degradation of DNA was observed after incubation of 1 µg of pUC19 DNA with 25 µL of PCR ROMAX (2X) in 50 µL of reaction mixture for 4 hours at 37°C and at 70°C.

• Exodeoxyribonuclease Assay

No detectable degradation of DNA was observed after incubation of 1 μg of lambda DNA/HindIII fragments with 25 μL of ROMAX (2X) in 50 μL of reaction mixture for 4 hours at 37°C and at 70°C.

• Ribonuclease Assay

No contaminating RNase activity was detected after incubation of 1 µg of [3H]-RNA with 25 µL ROMAX (2X) in 50 µL of reaction mixture for 4 hours at 37°C and at 70°C.

• Functional Assay

ROMAX (2X) was tested for amplification of 597 bp and 268 bp single copy genes from human genomic DNA.

ROMAX 2X PCR Master Storage

ROMAX 2X PCR Master: Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease and Prime-RNase A at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the inner surface of the kit lid.

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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