MiRJia PB Kit, (NO RBC Lysis Buffer)

 22,342,320 40,216,176

MiRJia PB Kit uses silica technology and is formulated for rapid and convenient isolation of up to 50 µg miRNA and total RNA (≥18 nucleotides) from whole blood and PBMC. Up to 10 ml blood can be processed with this product. This Kit provides the possibility of miRNArich fraction isolation. This product contains phenol.

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Description

Kit content

Component 50 preps 100 preps
RNSol Reagent 50 ml 100 ml
TWB1 (concentrate) 17 ml 35 ml
TWB2 (concentrate) 15 ml 27.5 ml
Nuclease free RBC Lysis Buffer

Just supplied in MiRJia PB Kit with RBC Lysis Buffer

2.5 L 5 L
Nuclease-free Water 5 ml 10 ml
Spin Column 50 100
Collection Tube 50 100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, RNSol Reagent and nuclease free RBC Lysis buffer should be kept dry, at 2-8 °C and all remaining reagent should be kept at room temperature (15-25 °C). When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended use of MiRJia PB Kit (NO RBC Lysis Buffer)

MiRJia PB Kit (NO RBC Lysis Buffer) provides the procedures necessary for purifying total RNA from peripheral blood mononuclear cell. Notice that, MiRJia PB Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

MiRJia PB Kit (NO RBC Lysis Buffer) is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Procedure

The MiRJia PB Kit is designed for isolating all forms of RNA molecules from up to 1×107 PBMCs. Fresh or frozen samples should be lysed and homogenized. Aqueous phase is separated using chloroform. Then, RNA binding to the silica membrane is achieved selectively, by the addition of Ethanol to the lysate. Contaminants are removed by two specific kind of washing buffers. Pure miRNA and total RNA is finally eluted in nuclease free water. Isolated RNA (miRNA or total RNA) is ready to use in downstream applications. It has A 260/ A 280 ratios of 1.9–2.3 by spectrophotometer, confirming high purity.

Equipment & Reagents to be supplied by user

  • Absolute Ethanol
  • 1.5 ml RNase free microcentrifuge tubes
  • Pipets and RNase free pipet tips
  • Benchtop refrigerated microcentrifuge
  • Vortex
  • TissueLyser/ Mortar and pestle/ Homogenizer
  • Syringe and needles

Applications

The isolated RNA can be used in many downstream applications:

  • RNA Sequencing
  • cDNA synthesis
  • Poly A+ RNA selection
  • Microarrays
  • RT-PCR and real-time RT-PC
  • Northern, dot blot analysis

Recommended starting material:

To reach optimized results it is better to follow as listed below. The size of the recommended material to use with determined RNSol and chloroform amount are written in Table 2.

Table 2. Appropriate sample size and amount of RNSol, Chloroform and Ethanol amount

Whole blood RNSol Amount Chloroform Amount Ethanol Amount
1 ml to 3 ml 500 µl 300 µl 400 µl
3 ml to 5 ml 700 µl 400 µl 400 µl
5 ml to 10 ml 1 ml 500 µl 400 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

100 prep, 50 prep

Specification

Features Specifications
Elution Volume 30-100µl
Technology Silica Technology
Main Sample Type ·         PBMC

·         WBC

·         Whole blood

Processing Manual
Purification of Total RNA, Mirna, Poly A+ Mi-RNA, DNA Or Protein Yes
Sample Amount Up to 107 cells
Operation Time Per Reaction Less Than 60 Min
Typical Yield 100 µg
Average Purity A260/A280= 1.9-2.3
Size of RNA Purified Total RNA
Enzyme No

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Protocol

none!

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Resources

MSDS MiRJia PB kit, No RBC

Hand Book MiRJia PB kit, No RBC

Quick Protocol EN  FA

Video Protocol Download

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