Hy-Fidelity Pfu Polymerase, Recombinant

 16,900,000 67,620,000

Hy-Fidelity Pfu Polymerase is an engineered version of pfu DNA polymerase with enhanced yield and higher fidelity. It possesses a proofreading 3’-5’ exonuclease activity.

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Description

Hy-Fidelity Pfu Polymerase, Recombinant content

Component 250 Reactions 500 Reactions
Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl) 100 µl 200 µl
PCR Buffer 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase 400 µl 800 µl

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Intended Use

Notice that, these products are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.

Quality Control

All ROJETechnologies products are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.

Description

Hy-Fidelity Pfu Polymerase is an engineered version of pfu DNA polymerase with enhanced yield and higher fidelity. It possesses a proofreading 3’-5’ exonuclease activity.

Equipment & Reagents to be supplied by user

  • Pipets and pipet tips
  • Microcentrifuge tube
  • Thermal cycler
  • Mineral oil (for thermal cyclers without a heated lid)
  • Primers

Prepare PCR Reactions

It is recommended to prepare PCR reactions as indicated in Table2.

Table 2. Prepare PCR Reactions, Hy-Fidelity Pfu PCR Set

Component Volume
Template Variable
Forward primer (10 µM) 1 µl
Reverse primer (10 µM) 1 µl
Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl) 1 µl
PCR Buffer, 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase 4 µl
HiPure-dNTPs mix, 2.5 mM 5-8 µl
Water for Molecular Biology Up to 50 µl

 

Tips for Optimizing PCR Reaction

  • A final concentration of 2mM MgSO4 is sufficient for most targets amplification. For some targets, more Mg2+ may be required; use the 50 mM MgSO4 stock to test from 2 mM to 4 mM (final concentration) in 0.25 mM increments

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]

 

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Additional information

Size

1000 unit, 250 unit

Specification

Feature Hy-ROJE PCR Set  Hy-Fidelity Pfu PCR S
Fidelity 1 2 54
Expression rate 6 kb/min 2-4 kb/min
Amplification of genomic DNA fragment up 4 kb 15 kb
Amplification of plasmid DNA fragment up __ 20 kb
Hot-start No Yes

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Protocol

none!

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Resources

MSDS Hy-Fidelity Pfu Polymerase, Recombinant

Hand Book Hy-Fidelity Pfu Polymerase, Recombinant

Quick Protocol EN  FA

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