Hy-Fidelity Pfu Polymerase, Recombinant content
|Component||250 Reactions||500 Reactions|
|Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl)||100 µl||200 µl|
|PCR Buffer 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase||400 µl||800 µl|
Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.
Notice that, these products are intended for molecular biology applications not for diagnostic use. We recommend all users to study RNA experiments guideline, before starting their work.
Guarantee & Warranty
ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com.
All ROJETechnologies products are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing REF and Lot number on web at www.rojetechnoloes.com.
Hy-Fidelity Pfu Polymerase is an engineered version of pfu DNA polymerase with enhanced yield and higher fidelity. It possesses a proofreading 3’-5’ exonuclease activity.
Equipment & Reagents to be supplied by user
- Pipets and pipet tips
- Microcentrifuge tube
- Thermal cycler
- Mineral oil (for thermal cyclers without a heated lid)
Prepare PCR Reactions
It is recommended to prepare PCR reactions as indicated in Table2.
Table 2. Prepare PCR Reactions, Hy-Fidelity Pfu PCR Set
|Forward primer (10 µM)||1 µl|
|Reverse primer (10 µM)||1 µl|
|Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl)||1 µl|
|PCR Buffer, 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase||4 µl|
|HiPure-dNTPs mix, 2.5 mM||5-8 µl|
|Water for Molecular Biology||Up to 50 µl|
Tips for Optimizing PCR Reaction
- A final concentration of 2mM MgSO4 is sufficient for most targets amplification. For some targets, more Mg2+ may be required; use the 50 mM MgSO4 stock to test from 2 mM to 4 mM (final concentration) in 0.25 mM increments
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, [email protected]
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