Hy-Fidelity Pfu Polymerase, Recombinant

 9,944,000 39,776,000

Pfu DNA polymerase is an engineered version of pfu DNA polymerase. It is a fast, high fidelity and high processivity hot start DNA polymerase.

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Description

Hy-Fidelity Pfu Polymerase, Recombinant content

Component25 Reactions100 Reactions 500 Reactions
Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl)25 unit

(10 µl)

250 unit

(100 µl)

500 unit

(200 µl)

PCR Buffer, 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase100 µl400 µl2 x 1 ml

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all components should be kept dry, at -20 °C. When storage condition is as directed, all components are stable until expiration date, as indicated on the kit box.

Intended Use of Hy-Fidelity Pfu Polymerase

Notice that, these products are intended for molecular biology applications not for diagnostic use.  We recommend all users to study RNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

All ROJETechnologies products are tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Prepare PCR Reactions

It is recommended to prepare PCR reactions as indicated in Table2.

Table 2. Prepare PCR Reactions, Hy-Fidelity Pfu PCR Set

Component Volume
TemplateVariable
Forward primer (10 µM)1 µl
Reverse primer (10 µM)1 µl
Hy-Fidelity Pfu Polymerase, Recombinant, (2.5U/μl)1 µl
PCR Buffer, 10X, with MgSO4, Optimized for Hy-Fidelity Pfu Polymerase4 µl
HiPure-dNTPs mix, 2.5 mM5-8 µl
Water for Molecular BiologyUp to 50 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

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Additional information

Size

1000 unit, 250 unit

Specification

This post is also available in: Persian

Protocol

none!

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Resources

MSDS Hy-Fidelity Pfu Polymerase, Recombinant

Hand Book Hy-Fidelity Pfu Polymerase, Recombinant

Quick Protocol EN  FA

This post is also available in: Persian