Description
Kit content
| Component | 50 preps |
| RBC Lysis Buffer, DNSol Edition | 100 ml |
| ROS | 25 ml |
| RRB | 5 ml |
| Prime RNase A | 250 µl |
| RJ-Protease | 1 ml |
Storage
Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept at room temperature except RJ-Protease and Prime RNase A. store these enzymes at -20 ̊C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on label box.
Intended Use
The DNSol Clotted Blood Kit provides the components and procedures necessary for purifying genomic DNA from clotted blood. Notice that, DNSol Clotted Blood Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.
Warning and Precautions
Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at ROJE Technologies
Quality Control
DNSol Clotted Blood Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. The results of all experiments are accessible by addressing Cat and Lot number on web at ROJE Technologies
Description
DNA isolation from clotted blood can be difficult and often results in low yields. However, ROJETechnologies provides DNSol Clotted Blood Kit, a timesaving, reliable and meticulous method for high quality genomic DNA isolation from Clotted blood. DNSol Clotted Blood Kit is manual and based on Solution for isolation of concentrated, highly purified, intact genomic DNA, which is suitable to be used for a variety of downstream applications such as PCR analysis and restriction endonuclease digestions. The solution based system minimizes DNA fragmentation that may be problematic in spin-column / filtration based methods.
Procedure
In DNSol Clotted Blood Kit, WBCs are separated after lysing red blood cells by RBC Lysis buffer, then WBCs are incubated with RJ-Protease and ROS Buffer. Aqueous phase is separated using phenol-chloroform. Aqueous phase is used in order to obtain a pure nucleic acid. DNA will be precipitated using isopropanol and washed away by using 70% ethanol to eliminate salt contaminations. Finally, DNA is rehydrated using RRB. Purified DNA has A260/A280 ratios approximately 1.7-2 and A260/A230 ratios of 1.8-2.2. The rehydrated DNA is stable for several months at 4 °C and greater than 1 year at -20 °C.
Equipment & Reagents to be supplied by user
- Ethanol (96-100%)
- Phenol
- Chloroform
- Isopropanol
- Pipets and pipet tips
- 5 ml Microtube
- Vortex
- Centrifuge
- Micro centrifuge
- Dry Heat Block/ Water Bath
Applications
The isolated DNA can be used in many downstream applications:
- Different kinds of PCRs, including Long-range PCR
- Sequencing
- Restriction digestion
- Southern blotting
- Cloning
Technical Assistance
ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.
Contact our technical support at any time by selecting one of these ways:
- Through our telephone and fax number available at ROJETechnologies website.
- You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)
Or send your questions to this email address, [email protected]
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