DNJia Virus DNA Kit

Description

Kit content

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature except RJ-Protease. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

DNJia Virus DNA Kit provides the components and procedures necessary for purifying viral DNA from cell-free samples such as body fluid, serum, plasma and animal tissue. Notice that, DNJia Virus DNA Kit is intended for molecular biology applications not for diagnostic use. We recommend all users to study DNA experiments guideline, before starting their work.

Guarantee & Warranty

ROJETechnologies guarantees the efficiency of all manufactured kits and reagents. For more information on choosing proper kits based on your needs, please contact our technical support team. If any product does not satisfy you, due to reasons other than misuse, please contact our technical support team. If problem is due to manufacturing process, ROJE team will replace the Kit for you.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at www.rojetechnologies.com .

Quality Control

DNJia Virus DNA Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at www.rojetechnoloes.com.

Description

The DNJia Virus DNA Kit is designed for rapid and efficient purification of high-quality viral DNA from various human and animal liquid samples such as body fluid, plasma, serum etc. The kit utilizes a silica-based membrane technology in the form of a convenient spin column for nucleic acid isolation. DNJia Virus DNA Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA can be directly used in PCR, qPCR or other nucleic acid-based assays.

Procedure

DNJia Virus DNA Kit is designed for isolating both viral DNA from body fluid, serum, plasma etc. Lysis is achieved by incubation of the sample in GLB; and in a RJProtease enzyme solution. Appropriate conditions for DNA binding to the silica membrane is achieved by the addition of ethanol to the lysate. Then, DNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure viral DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. A symmetric peak at 260 nm by spectrophotometer, confirms high purity of isolated nucleic acid.

Equipment & Reagents to Be Supplied by User

• Molecular biology grade ethanol (96-100%)
• Sterile, RNase-free pipets and pipet tips
• 1.5 ml Microtube
• Vortex
• Centrifuge and Micro centrifuge
• Dry Heat Block/ Water Bath

Applications

The isolated DNA/RNA can be used in many downstream applications:

• Different kinds of PCRs
• Viral genotyping
• Viral detection

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

• Through our telephone and fax number available at ROJETechnologies website.
• You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, [email protected]