DNJia VirAll Kit

 24,600,000

The DNJia VirAll Kit is designed for rapid and efficient purification of high-quality viral DNA from various human and animal liquid samples such as body fluid, plasma, serum etc. The kit utilizes a silica-based membrane technology in the form of a convenient spin column, which takes less than 30 minutes. DNJia VirAll Kit needs less handling and it is convenient for simultaneous isolation, which makes it favorites for laboratory with many isolations in a day. The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA can be directly used in PCR, qPCR or other nucleic acid-based assays.

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Description

Kit content

Component100 preps
GLB22 ml
BWB1 (concentrate)35 ml
BWB2 (concentrate)16.5 ml
ERR20 ml
RJ-Protease2 x 1.25 ml
Carrier RNA610  μg
HiPure VI Column100
Collection Tube100

Storage

Shipment condition is checked by ROJETechnologies. After arrival, all reagents should be kept dry, at room temperature. We suggested to store RJ-Protease at -20 °C for longer stability; However, for routine use it could be stored at 2-8 °C. When storage condition is as directed, all reagents are stable until expiration date, as indicated on the kit box.

Intended Use

DNJia VirAll Kit provides the components and procedures necessary for purifying viral DNA/RNA from cell-free samples such as body fluid, serum, plasma etc. Notice that, DNJia VirAll Kit is intended for molecular biology applications not for diagnostic use.  We recommend all users to study DNA experiments guideline, before starting their work.

Warning and Precautions

Due to chemical material usage that may be hazardous, always make sure to wear suitable lab coat, disposable gloves, and protective eyewear. Material Safety Data Sheet (MSDS) for all products and reagents are provided. They are accessible online at rojetechnologies.com

Quality Control

DNJia VirAll Kit is tested against predetermined experiments on a lot-to-lot basis according to ROJETechnologies ISO-certified quality management system, to ensure consistent product quality. For your information, the results of all experiments are accessible by addressing Cat and Lot number on web at rojetechnoloes.com

Procedure

DNJia VirAll Kit is designed for isolating both DNA and RNA from body fluid, serum, plasma and etc. Lysis is achieved by incubation of the sample in a RJ-Protease enzyme solution and GLB. Appropriate conditions for DNA and RNA binding to the silica membrane is achieved by the addition of Ethanol to the lysate. Then, DNA or RNA is selectively bound to the membrane. Contaminants are removed by two specific washing buffers. Pure viral DNA is finally eluted in rehydration buffer. Isolated DNA is ready to use in downstream applications. It has A 260/A 280 ratios of 1.7-2, and a symmetric peak at 260 nm by spectrophotometer, confirms high purity.

Equipment & Reagents to be supplied by user

  • Ethanol (96-100%)
  • Sterile, RNase-free pipets and pipet tips
  • 5 ml Microtube
  • Vortex
  • Centrifuge and Micro centrifuge
  • Dry Heat Block/ Water Bath

Applications

The isolated DNA can be used in many downstream applications:

  • Different kinds of PCRs
  • Restriction enzyme digestion
  • Viral genotyping
  • Viral detection
  • Viral load monitoring

Recommended starting material:

The size of recommended starting material to use with determined lysis volume are listed here.

Table 2: Recommended starting material and Lysis Buffer amount

SampleSize of Starting materialLysis Buffer Amount
Plasma/serumUp to 200 µl200 µl

Technical Assistance

ROJETechnologies guarantees your complete satisfaction. ROJE technical support team composed of highly trained experienced scientists, who are able to troubleshoot most problems you face. Our technical support team can offer expert advice which may help you select suitable product.

Contact our technical support at any time by selecting one of these ways:

  • Through our telephone and fax number available at ROJETechnologies website.
  • You can submit your question directly to ROJE Technical Support Team from our website (rojetechnologies.com)

Or send your questions to this email address, Technicalsupport@Rojetechnologies.com

 

Additional information

Size

100 reactions

Specification

FeaturesSpecifications
Elution volume20-150 µl
TechnologySilica technology
Main sample typeBody Fluid, serum, Plasma
Processing Manual
Sample amountUp to 250 µl
Biomolecule isolationDNA and RNA
Operation time per reactionLess than 30 Min
Typical yieldVaries
Carrier RNANot provided in the kit
EnzymeRJ-Protease

Protocol

none!

Resources

MSDS DNJia Viral Kit

Hand Book DNJia VirAll Kit

Quick Protocol EN  FA

Video Protocol Download