HLA B27 Diagnostic Kit, SSP-PCR assay ﷼ 5,016,000 – ﷼ 9,028,800 HLA B-27 Molecular Diagnostic Kit is designed to determine HLA B-27 alleles based on the Polymerase Chain Reaction method using sequence specific primer (PCR-SSP assay), which enables amplification of defined DNA sequences. The principle of the SSP assay is to generate an amplicon only when the sequence of a primer is perfectly complementary to the target sequence of a DNA sample. On the other hand, non-complementary primer do not bind to the DNA and then no amplification takes place. Evaluation of the result is performed by agarose gel electrophoresis. In the electric field the amplicons separate according to their size. If no amplification takes place, no specific band is seen. This kit is tested for 1094 individuals and has shown 100% concordance compared to sequencing, showing 100% specificity and sensitivity in this population size.